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A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

Chen, Kai and Ma, Biao and Li, Jiali and Chen, Erjing and Xu, Ying and Yu, Xiaoping and Sun, Chuanxin and Zhang, Mingzhou (2021). A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens. International journal of environmental research and public health. 18 , 4574
[Research article]

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Abstract

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coli O157:H7. In this work, the entire experimental process could be completed in 20 min at 37 degrees C. The limits of detection (LODs) of EuNP-based LFIA-RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coli O157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA-RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9-114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA-RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

Authors/Creators:Chen, Kai and Ma, Biao and Li, Jiali and Chen, Erjing and Xu, Ying and Yu, Xiaoping and Sun, Chuanxin and Zhang, Mingzhou
Title:A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens
Series Name/Journal:International journal of environmental research and public health
Year of publishing :2021
Volume:18
Article number:4574
Number of Pages:14
Publisher:MDPI
ISSN:1661-7827
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Public Health, Global Health, Social Medicine and Epidemiology
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Microbiology in the medical area
Keywords:recombinase polymerase amplification, europium nanoparticles, lateral flow immunoassay, multiplex detection, food-borne pathogens
URN:NBN:urn:nbn:se:slu:epsilon-p-111980
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111980
Additional ID:
Type of IDID
DOI10.3390/ijerph18094574
Web of Science (WoS)000650270100001
ID Code:23904
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:27 May 2021 10:23
Metadata Last Modified:27 May 2021 10:31

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