Home About Browse Search
Svenska


Use of natural plant extracts as a novel microbiological quality indicator in raw milk: An alternative for resazurin dye reduction method

Madushan, Ranga and Vidanarachchi, Janak K. and Prasanna, P. H. P. and Werellagama, Shanika and Priyashantha, Hasitha (2021). Use of natural plant extracts as a novel microbiological quality indicator in raw milk: An alternative for resazurin dye reduction method. Food Science and Technology. 144 , 111221
[Research article]

[img] PDF
4MB

Abstract

In the present study, we propose a novel field-scale analytical tool for rapid screening of microbiological quality in raw milk using aqueous extracts of plant materials, i.e. flowers (e.g. Hibiscus rosa-sinensis L. and Clitoria ternatea), taproots (e.g. Beta vulgaris) and pricklypears (e.g. Opuntia dillenii). For each plant extract, the colour changes in raw milk were evaluated between 4.5 and 6.5 pH against the resazurin dye as a control. The plant anthocyanin content in each extract was analyzed by the differential pH method using a spectrophotometer. The Hibiscus rosa-sinensis flower extract was opted to further test since it had a pH-sensitive colour change (6.5; maroon to 6.2; light-pink) compared to other plant extracts, which did not indicate a noticeable colour variation with pH. Anthocyanin content of the Hibiscus extract was 0.59 g/mL. The novel method showed high linearity (R-2 = 0.95), 100% accuracy and greater repeatability with an intermediate level of precision. The limit of quantification and detection was 0.46 and 0.15 g/mL, respectively. In conclusion, we demonstrated the potential in using water extract of Hibiscus rosa-sinensis L. flowers as an alternative to the resazurin dye reduction method for rapid and accurate microbiological quality control for raw milk procurement in remote areas.

Authors/Creators:Madushan, Ranga and Vidanarachchi, Janak K. and Prasanna, P. H. P. and Werellagama, Shanika and Priyashantha, Hasitha
Title:Use of natural plant extracts as a novel microbiological quality indicator in raw milk: An alternative for resazurin dye reduction method
Series Name/Journal:Food Science and Technology
Year of publishing :2021
Volume:144
Article number:111221
Number of Pages:9
Publisher:ELSEVIER
ISSN:0023-6438
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Keywords:Anthocyanin, Plant aqueous extracts, Microbiological safety, Milk keeping quality, Platform test
URN:NBN:urn:nbn:se:slu:epsilon-p-112001
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112001
Additional ID:
Type of IDID
DOI10.1016/j.lwt.2021.111221
Web of Science (WoS)000642470800003
ID Code:23917
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:27 May 2021 13:03
Metadata Last Modified:27 May 2021 13:11

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits