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Functional studies of the role of plant dehydrins in tolerance to salinity, desiccation and

Svensson, Jan (2001). Functional studies of the role of plant dehydrins in tolerance to salinity, desiccation and. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae. Agraria, 1401-6249 ; 259
ISBN 91-576-5779-3
[Doctoral thesis]

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Dehydrins are a group of proteins believed to play a fundamental role in plant response and adaptation to abiotic stresses that lead to cellular dehydration. This group of proteins can be found in seeds in the later maturation stage and the desiccated seed, and are induced by salt, drought and low temperature stress. Dehydrins share no sequence homology with any known protein or enzyme and have so far only been found in photosynthesizing organisms. We have purified four recombinant dehydrins (RAB18, LTI29, LTI30 and COR47) and introduce a method previously not used for purification of dehydrins; immobilized metal ion affinity chromatography. Furthermore the accumulation of both transcript and protein was analyzed for five dehydrins (ERD14, RAB18, LTI29, LTI30 and COR47). Based on the accumulation data the dehydrins could be divided into four groups: (I) LTI30 cold specific, (II) RAB18 ABA regulated, (III) ERD14 constitutive and (IIII) LTI29 and COR47 mainly cold induced. Immunolocalization studies of four dehydrins (ERD14, LTI29, LTI30 and RAB18) showed tissue and cell type specific accumulation in unstressed plants. ERD14 and LTI29 were detected in the root tip and the vascular tissue whereas RAB18 was detected in stomatal guard cells. LTI30 was not detected in unstressed plants. In stress treated plants RAB18, LTI29 and ERD14 were detected in most cells whereas LTI30 was only detected in the vascular tissue and in pollen sacks. Ectopic expression of multiple dehydrins in Arabidopsis resulted in improved survival of plants exposed to drought and freezing stress. In addition, salt stressed seeds overproducing DHNs showed improved germination/cotyledon emergence. We isolated a dehydrin gene (DHNA) from Physcomitrella patens which shared some characteristics of dehydrins cloned in higher plants. DHNA was found to be expressed during salt and water stress and by ABA treatm

Authors/Creators:Svensson, Jan
Title:Functional studies of the role of plant dehydrins in tolerance to salinity, desiccation and
Series Name/Journal:Acta Universitatis Agriculturae Sueciae. Agraria
Year of publishing :January 2001
Number of Pages:47
ALLI. Svensson, J., Palva, E. T. and Welin, B. 2000. Purification of Recombinant Arabidopsis thaliana Dehydrins by Metal Ion Affinity Chromatography. Prot. Exp. and Purif. 20: 169-178. II. Nylander, M., Svensson, J., Palva, E. T. and Welin, B. 2001. Stress-induced accumulation and tissue specific localization of Dehydrins in Arabidopsis thaliana. Plant Mol. Biol. In press. III. Puhakainen, T., Svensson, J., Mäkelä, P., Niklander-Teeri, V., Heino, P. and Palva, E. T. Ectopic expression of dehydrin genes enhances tolerance to freezing and drought stress in Arabidopsis. Manuscript. IV. Svensson, J. and Welin, B. Cloning and Characterization of a drought-and salt-induced dehydrin-like gene in Physcomitrella patens. Manuscr
Place of Publication:Uppsala
ISBN for printed version:91-576-5779-3
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
Subjects:Not in use, please see Agris categories
Agrovoc terms:plant physiology, stress, frost, cold, dehydration, adaptation, aba, proteins, temperature resistance, gene expression
Keywords:dehydrins, low temperature, drought, ABA, salinity, protein purification, Arabidopsis, Physcomitrella patens
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ID Code:24
Department:?? 4062 ??
Deposited By: Staff Epsilon
Deposited On:29 Apr 2002 00:00
Metadata Last Modified:02 Dec 2014 10:01

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