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Hormones and sex steroid receptors in the female pig

effects of mating, artificial insemination, seminal plasma and genistein

Norrby, Mattias (2010). Hormones and sex steroid receptors in the female pig. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:71
ISBN 978-91-576-7516-3
[Doctoral thesis]

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Abstract

Artificial insemination (AI) of sows and gilts is replacing mating in many countries. Another change over the last decades is that soya, rich in phytooestrogens, is routinely used in the diet to pigs. The aims of this thesis were to compare hormonal changes in blood plasma of the female pig during mating and insemination and to investigate if the phytooestrogen genistein influenced the hormonal responses and sex steroid receptor expression in the reproductive tract of inseminated gilts. Blood samples were taken via permanent catheters around mating or AI for analyses of hormones. Uterine and cervical cells were used in vitro to determine the role of seminal plasma. Three days after AI, gilts were euthanized and uterine and cervical tissues were prepared for morphological and immunohistochemical examination. Plasma cortisol concentration increased more during mating than AI in sows, in contrast prostaglandin F2α metabolite (PGFM) increased only after AI. By incubating cells from porcine cervix and uterus with seminal plasma prostaglandin synthesis was inhibited in a dose dependent manner, providing a possible explanation for the difference in PGFM after mating. When a feed relevant dose of genistein was added to gilts from 3 days before oestrus until 3 days after AI, prostaglandin F2α exhibited more frequent pulses with higher amplitudes after AI, the PGE2 level was elevated and plasma oxytocin was almost doubled. PGFM, however, had a similar pattern in both groups. Nevertheless gilts administered genistein showed no significant difference in cell proliferation or the expression of oestrogen receptors (ER) α and β or progesterone receptor in uterus and cervix. However, intra-treatment evaluation and correlations indicated that genistein may modulate receptor patterns and induce subtle morphological changes. In view of the results presented in this thesis it is surprising that the difference in reproductive performance is not greater between AI and mating, especially with the addition of soya to the diet.

Authors/Creators:Norrby, Mattias
Title:Hormones and sex steroid receptors in the female pig
Subtitle:effects of mating, artificial insemination, seminal plasma and genistein
Year of publishing :2010
Volume:2010:71
Number of Pages:69
Papers/manuscripts:
NumberReferences
ALLI Norrby M., Madsen MT., Borg Alexandersen C., Kindahl H., Madej A. (2007). Plasma concentrations of cortisol and PGF2α metabolite in Danish sows during mating, and intrauterine and conventional insemination. Acta Vet Scand 49 (36). II Madej M., Norrby M., Madsen MT., Johannisson A., Hansen C., Madej A. Effect of boar seminal plasma on production of prostaglandins and interleukin-6 from porcine endometrial and cervical cells and bovine endometrial cells (Manuscript). III Norrby M., Madsen MT., Saravia F., Lundeheim N., Madej A. (2010) Genistein alters the release of oxytocin, prostaglandins, cortisol and LH during insemination in gilts. Reprod Dom Anim. doi: 10.1111/j.1439-0531.2010.01669.x IV Norrby M., Madej A., Ekstedt E., Holm L. Effects of genistein on oestrogen and progesterone receptor, proliferative marker Ki-67 and carbonic anhydrase localization in the uterus and cervix of gilts after insemination (Manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7516-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:swine, oestrous cycle, artificial insemination, genistein, prostaglandins, oxytocin, endocrinology, evaluation, sweden
Keywords:Pig, oestrus, insemination, genistein, endocrinology, prostaglandin, oxytocin, oestrogen receptors, seminal plasma.
URN:NBN:urn:nbn:se:slu:epsilon-3121
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3121
ID Code:2401
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: Mattias Norrby
Deposited On:11 Nov 2010 00:00
Metadata Last Modified:02 Dec 2014 10:18

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