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Applications of viral metagenomics in the veterinary field

looking for the unknown

Blomström, Anne-Lie (2010). Applications of viral metagenomics in the veterinary field. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:85
ISBN 978-91-576-7530-9
[Doctoral thesis]

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Abstract

Viral metagenomics provide a powerful technology to investigate the viral flora of healthy and sick animals. Using these methodologies, we gain a better understanding in the etiology of diseases, as well as deepen our knowledge into the viruses circulating in nature and the complex interaction between virus and host. The aim of this thesis was to utilize viral metagenomics into different areas of interest for veterinary science. One multifactorial disease complex was studied, as were a disease of unknown etiology, where traditional methods had failed to identify a causative agent. The final study was on viruses in an arthropod vector. Postweaning multisystemic wasting syndrome (PMWS) was the multifactorial disease chosen here. Though, porcine circovirus type 2 (PCV-2) has been found the causative agent other factors, such as viruses, are believed to influence the disease. Using multiple displacement amplification followed by large-scale sequencing we discovered, apart from PCV-2 and torque teno virus, a novel porcine bocavirus in the background of PCV-2 in lymph nodes collected from pigs suffering from PMWS. This co-infection was seen in a high percentage (71%) of pigs suffering from PMWS compared to only 33% in pigs without PMWS. Shaking mink syndrome (SMS) was used to investigate a disease of unknown etiology. By random amplification and large-scale sequencing we found an astrovirus in the brain of minks experimentally infected with brain homogenate from diseased animals. Astrovirus was also detected in the brain of naturally infected minks. Vectors are important transmitters of disease, and in the final study, soft ticks (Ornithodoros) collected from a warthog burrow in Uganda were investigated for the presence of viruses. Among others, we discovered a possible novel RNA virus that showed a distant relationship to hepatitis E virus. In conclusion, viral metagenomics have successfully been applied to investigate three important areas for veterinary science and through these studies three novel viruses were discovered and genetically characterized.

Authors/Creators:Blomström, Anne-Lie
Title:Applications of viral metagenomics in the veterinary field
Subtitle:looking for the unknown
Series/Journal:Acta Universitatis agriculturae Sueciae
Year of publishing :2010
Volume:2010:85
Number of Pages:57
Papers/manuscripts:
NumberReferences
I.Blomström, A-L, Belák, S., Fossum, C., McKillen, J., Allan, G., Wallgren, P. & Berg, M. (2009). Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Research 146(1-2), 125-129.
II.Blomström, A-L, Belák, S., Fossum, C., Fuxler, L., Wallgren, P. & Berg, M. (2010). Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Virus Research 152(1-2), 59-64.
III.Blomström, A-L, Widén, F., Hammer, A-S., Belák, S. & Berg, M. (2010). Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome using viral metagenomics. (Manuscript accepted to be published in Journal of clinical microbiology; doi:10.1128/JCM.01940-10).
IV.Blomström, A-L, Ståhl, K., Masembe, C., Okurut, A. R., Atmned, P., Kempe, S., Bishop, R., Belák, S. & Berg, M. (2010). Viral metagenomic analysis of soft ticks (Ornithodoros) in Uganda reveals a novel RNA virus with distant relationship to hepatitis E virus. (Manuscript)
Place of Publication:Uppsala
Publisher:Dept. of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-7530-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:viroses, viruses, genomes, disease control, animal health
Keywords:viral metagenomics, postweaning multisystemic wasting syndrome, shaking mink syndrome, soft ticks (Ornithodoros), bocavirus, astrovirus
URN:NBN:urn:nbn:se:slu:epsilon-3127
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3127
ID Code:2402
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Anne-Lie Blomström
Deposited On:15 Nov 2010 00:00
Metadata Last Modified:02 Dec 2014 10:18

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