Home About Browse Search
Svenska


Exposure of zebrafish to brominated environmental chemicals

studies on reproduction, maternal transfer and early life-stage development

Norman Haldén, Anna (2010). Exposure of zebrafish to brominated environmental chemicals. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:97
ISBN 978-91-576-7542-2
[Doctoral thesis]

[img]
Preview
PDF
659kB

Abstract

Fish reproduction and embryonic development may be sensitive to environmental chemicals. This thesis investigated effects of brominated flame retardants (BFRs) and polybrominated dibenzo-p-dioxins (PBDDs) on reproduction and early life-stage development in zebrafish (Danio rerio). Most of these compounds are highly hydrophobic, hence it was hypothesized that important routes of exposure are dietary uptake and maternal transfer. Adult zebrafish were exposed to feed spiked with a mixture of structurally diverse BFRs to investigate accumulation from feed, maternal transfer, and effects on reproduction and early life-stage development. One of the compounds in the BFR mixture, i.e. 2,4,6-tribromophenol, was tested separately. The BFRs were also tested individually in an embryo toxicity test, to screen for effects of waterborne BFRs on early life stages. To investigate effects of PBDDs on reproduction, early life-stage development, and on the aryl hydrocarbon receptor (AHR) pathway, adult zebrafish were exposed to feed spiked with 2,3,7,8-tetraBDD (TBDD), or a mixture of PBDDs that was designed to reflect relative concentrations found in Baltic Sea biota. Most brominated chemicals exposed via feed were detected in females and in their offspring. Ovarian morphology was altered in all studies, and the PBDDs induced AHR-regulated genes and ethoxyresorufin-O-deethylase activity. Effects on early life-stage development were seen after parental and water exposure, although at concentrations generally higher than in the environment. However, compared to several other fish species, zebrafish have a relatively low sensitivity to AHR agonists regarding effects on early life stages. To be able to evaluate the risk of PBDDs for fish in the Baltic Sea, future studies should focus on fish species native in the Baltic Sea. Overall, the results suggest that maternal transfer is an important exposure route for several brominated environmental chemicals, and that these compounds may interfere with reproduction and early life-stage development in fish.

Authors/Creators:Norman Haldén, Anna
Title:Exposure of zebrafish to brominated environmental chemicals
Subtitle:studies on reproduction, maternal transfer and early life-stage development
Year of publishing :2010
Volume:2010:97
Number of Pages:67
Papers/manuscripts:
NumberReferences
I.Jenny Rattfelt Nyholm, Anna Norman, Leif Norrgren, Peter Haglund, Patrik L. Andersson (2008). Maternal transfer of brominated flame retardants in zebrafish (Danio rerio). Chemosphere 73(2), 203-208.
II. II. Anna Norman Haldén, Jenny Rattfelt Nyholm, Patrik L. Andersson, Leif Norrgren. Reproductive and developmental effects in zebrafish (Danio rerio) exposed to structurally diverse brominated flame retardants (manuscript).
III.Anna Norman Haldén, Jenny Rattfelt Nyholm, Patrik L. Andersson, Henrik Holbech, Leif Norrgren (2010). Oral exposure of adult zebrafish(Danio rerio) to 2,4,6-tribromophenol affects reproduction. Aquatic Toxicology 100(1), 30-37.
IV.Anna Norman Haldén, Kristina Arnoldsson, Peter Haglund, Anna Mattsson, Erik Ullerås, Joachim Sturve, Leif Norrgren. Retention and maternal transfer of brominated dioxins in zebrafish (Danio rerio) and effects on reproduction, aryl hydrocarbon receptor-regulated genes, and ethoxyresorufin-O-deethylase (EROD) activity (manuscript).
Place of Publication:Uppsala
Publisher:Dept. of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare
ISBN for printed version:978-91-576-7542-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L53 Animal physiology - Reproduction
T Pollution > T01 Pollution
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:fishes, reproduction, embryonic development, ovaries, histopathology, dioxins, pollutants, baltic sea
Keywords:zebrafish, reproduction, early life-stage development, maternal transfer, ovarian histopathology, brominated flame retardants, brominated dioxins, toxicity
URN:NBN:urn:nbn:se:slu:epsilon-3130
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3130
ID Code:2407
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Anna Norman
Deposited On:18 Nov 2010 00:00
Metadata Last Modified:02 Dec 2014 10:18

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits