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On the immune response in porcine enteric diseases

with special reference to swine dysentery, proliferative enteropathy and PMWS

Andersson, Märit (2010). On the immune response in porcine enteric diseases. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2010:88
ISBN 978-91-576-7533-0
[Doctoral thesis]

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Enteric infectious diseases are a common problem worldwide among pigs. It is important to gain a better knowledge about the pathogeneses of the diseases and the local immune response, in which the intestine has a very important role. The aim of this thesis was to study the immune response in pigs infected with Brachyspira hyodysenteriae, Lawsonia intracellularis or Porcine circovirus type 2 (PCV2) that cause swine dysentery (SD), proliferative enteropathy (PE) and postweaning multisystemic wasting syndrom (PMWS), respectively. For SD and PMWS, material from both experimental infections and field cases were used, whereas material from pigs with PE was available from field cases. The local immune response was studied by analysing the cytokine expression in intestines using the microarray and quantitative PCR techniques. For that purpose a porcine microarray with a limited number of cytokines representing different types of immune response was constructed and evaluated using in vitro stimulated porcine blood mononuclear cells, and porcine intestinal samples. A commercial available genome wide porcine cDNA microarray was applied for screening intestinal samples collected from experimental (PCV2) as well as field (Lawsonia intracellularis) studies. The serum analyses showed that IL-1β, IL-6 and TNF-α were increased during natural SD. In pigs with experimental PMWS, increased mRNA expressions in the intestine for IL-6, IL-10 and IFN-γ were found. The increased mRNA expression for IFN-γ was also observed in field cases. For pigs with PE no differences in cytokine levels in serum or intestine were found between control and case pigs. cDNA microarray screening of intestinal samples from pigs with PE indicated that an uncomplicated infection with L intracellularis does not evoke an immune response and that the severe clinical signs of haemorrhagic diarrhoea can be regarded as a complication to the chronic form. In PCV2-infected pigs a marked up-regulation of interferon-stimulated genes was noticed. Comparison of two different isolates of PCV2 revealed differences in gene expression evoked by the two isolates. Expanded analyses using methods established in the present thesis will provide valuable insight into immune reactions elicited at porcine enteric diseases.

Authors/Creators:Andersson, Märit
Title:On the immune response in porcine enteric diseases
Subtitle:with special reference to swine dysentery, proliferative enteropathy and PMWS
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2010
Number of Pages:80
ALLI. Jonasson, R., Andersson, M., Råsbäck, T., Johannisson, A., Jensen-Waern. (2006). Immunological alterations during the clinical and recovery phases of experimental swine dysentery. J Med Microbiol 55, 845-55. II. Andersson, M., Berg, M., Fossum, C., Jensen-Waern, M. (2007). Development of a microarray for studying porcine cytokine production in blood mononuclear cells and intestinal biopsies. J Vet Med A Physiol Pathol Clin Med 54, 161-8. III. Jacobson, M., Andersson, M., Lindberg, R., Fossum, C., Jensen-Waern, M. Microarray and cytokine analyses in pigs with diarrhoea and in healthy pigs in herds confirmed to be infected with Lawsonia intracellularis. Submitted. IV. Andersson, M., Ahlberg, V., Fossum, C. Intestinal gene expression in pigs experimentally infected with PCV2 (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7533-0
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:swine, intestinal diseases, diarrhoea, circoviridae, cytokines, gene expression, disease control, immunology
Keywords:pig, intestine, microarray, cytokine, Brachyspira hyodysenteriae, Lawsonia intracellularis, PCV2, gene expression
Permanent URL:
ID Code:2412
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Märit Andersson
Deposited On:23 Nov 2010 00:00
Metadata Last Modified:02 Dec 2014 10:18

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