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Role of oxidative stress and antioxidants in domestic and non-domestic cat spermatozoa

with special reference to cryopreservation

Thuwanut, Paweena (2010). Role of oxidative stress and antioxidants in domestic and non-domestic cat spermatozoa. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:70
ISBN 978-91-576-7515-6
[Doctoral thesis]

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Abstract

Living cells, including spermatozoa, stored under aerobic conditions require oxygen to support their normal metabolism. Excessive levels of metabolites (reactive oxygen species; ROS) can cause cell damage or lipid peroxidation. The ROS and lipid peroxidation can be detected during preparation and cryopreservation of mammalian spermatozoa. The negative effects of ROS can be partially ameliorated by the action of antioxidants. The aims of this thesis were to 1) study the effects of antioxidants on cryopreserved epididymal spermatozoa from the domestic cats and to apply the results from domestic cats to cryopreserved wild felid ejaculated spermatozoa (flat-headed cats; Prionailurus planiceps) in Thailand; 2) detect lipid peroxidation and its effects on post-thaw epididymal cat spermatozoa and 3) evaluate basic seminal characteristics of captive flat-headed cats. In the first study, the semen extender was supplemented with two types of non-enzymatic antioxidants (cysteine and a water soluble vitamin E analogue Trolox). Vitamin E had positive effects on post-thaw sperm motility, progressive motility and membrane integrity. In the second study, centrifuged post-thaw epididymal cat spermatozoa were resuspended in media with or without egg yolk and with or without a transition metal (Fe2+) (which induces lipid peroxidation). Lipid peroxidation was measured by using a lipophilic dye probe (BODIPY581/591 C11). The results demonstrated that BODIPY581/591 C11 could be used to detect lipid peroxidation in cat spermatozoa. This reaction did not significantly increase during incubation unless induced by Fe2+. In the third study, enzymatic antioxidants (catalase, glutathione peroxidase; GPx and superoxide dismutase) were added to the semen extender. Lipid peroxidation was induced after thawing. Supplementation of GPx resulted in positive effects on post-thaw motility, linear motility, mitochondrial activity, membrane and DNA integrity. Although antioxidants had positive effects on sperm quality, the concentration of antioxidants used in this study did not reduce lipid peroxidation. In the last study, the basic seminal traits of captive flat-headed cats were evaluated, as were the effects of adding the vitamin E and GPx to the semen extender for flat-headed cat spermatozoa. The results demonstrated that the captive flat-headed cats were affected by teratozoospermia. The GPx had positive effects on the post-thaw quality of flat-headed cat spermatozoa in a similar manner to domestic cat sperm quality but it did not significantly affect in vtiro heterologous fertility tests. In conclusion, oxidative stress might be one of the factors causing damage during preservation of domestic and non-domestic cat spermatozoa. Post-thaw sperm quality was enhanced by the action of antioxidants.

Authors/Creators:Thuwanut, Paweena
Title:Role of oxidative stress and antioxidants in domestic and non-domestic cat spermatozoa
Subtitle:with special reference to cryopreservation
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2010
Volume:2010:70
Number of Pages:72
Papers/manuscripts:
NumberReferences
I.Thuwanut P, Chatdarong K, Techakumphu M and Axnér E. The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology 70(2): 233-240, 2008.
II.Thuwanut P, Axnér E, Johanisson A and Chatdarong K. Detection of lipid peroxidation reaction in frozen-thawed epididymal cat spermatozoa by using BODIPY581/591 C11. Reprod Domest Anim Suppl 2: 373-376, 2009.
III.Thuwanut P, Chatdarong K, Johanisson A, Bergqvist AS, Söderquist L and Axnér E. Cryopreservation of epididymal cat spermatozoa: effects of in vitro antioxidative enzymes supplementation and lipid peroxidation induction. Theriogenology 73(8): 1076-1087, 2010.
IV.Thuwanut P, Chatdarong K, Bergqvist AS, Söderquist L, Thiangtum K, Tongthainan D and Axnér E. The effects of antioxidants on semen traits and in vitro fertilizing ability of spermatozoa from the flat-headed cat (Prionailurus planiceps). (Manuscript submitted for publication).
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Sciences
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare
ISBN for printed version:978-91-576-7515-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:cats, males, spermatozoa, semen, cryopreservation, viability, oxidative stress, antioxidants, domestic animals, wild animals
Keywords:Feline, Sperm cryopreservation, Oxidative stress, Antioxidant
URN:NBN:urn:nbn:se:slu:epsilon-3138
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3138
ID Code:2417
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Paweena Thuwanut
Deposited On:26 Nov 2010 00:00
Metadata Last Modified:02 Dec 2014 10:18

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