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Olfaction in the Spruce Bark Beetle, Ips typographus

receptor, neuron and habitat

Andersson, Martin (2011). Olfaction in the Spruce Bark Beetle, Ips typographus. Diss. (sammanfattning/summary) Alnarp, Sweden : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2011:4
ISBN 978-91-576-7573-6
[Doctoral thesis]

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Abstract

The bark beetle Ips typographus regularly kills spruce trees in the Palearctic. Spruces are colonized by means of attraction to an aggregation pheromone. Attraction is modulated by anti-attractive volatiles (NHV) from non-host plants. In this thesis, olfaction in I. typographus was studied. At the molecular level, putative odorant receptors (ORs) were identified. When compared with OR sequences of Tribolium castaneum, a drastic extension of bark beetle OR function was indicated. The ORs are situated in the membrane of olfactory receptor neurons (ORNs). By recording odor responses from these neurons, 17 ORN classes that strongly responded to pheromone, host, and non-host compounds, were characterized. Surprisingly, al¬most 25 % of these responded to anti-attractive NHV. The ORN for the essential pheromone compound cis-verbenol (cV), was in some sensilla co-localized with an ORN for the host plant compound 1,8-cineole (Ci). Ci inhibited pheromone attraction in the field. In addition, while the ORN for Ci responded, the response in the co-localized cV ORN was inhibited, indicating interactions between ORNs. In electrophysiological studies of olfaction, odor stimuli are often based on a known amount of compound put on a filter paper, but the amount in the vapor phase (the actual stimulus) is unknown. Using a photoionization detector, vapor amounts of compounds released from stimulus cartridges were measured. A large variation between compounds, solvents, and successive stimulations were recorded. This indicated that stimulus doses often must be corrected for differences in volatility, and that consistent test protocols are required. In nature, insects orient in semiochemically diverse habitats with intermixing odor plumes. We studied the attraction to separated pheromone components, and pheromone and anti-attractant sources in the bark beetle and in the moth Spodoptera littoralis. While the beetle responded to spacing distances of a few decimeters, the moth responded to only a few centimeters. This may reflect different processing mechanisms in the sex pheromone system of the moth as compared to the aggregation pheromone system of the beetle. In addition, a long-distance (at least 2 m) effect of NHV was found, indicating a potential for these anti-attractants in forest protection against the beetle.

Authors/Creators:Andersson, Martin
Title:Olfaction in the Spruce Bark Beetle, Ips typographus
Subtitle:receptor, neuron and habitat
Year of publishing :2011
Volume:2011:4
Number of Pages:51
Papers/manuscripts:
NumberReferences
ALLI Martin N. Andersson, Jonas M. Bengtsson, Ewald Grosse-Wilde, Marcus C. Stensmyr, Ylva Hillbur, Fredrik Schlyter, Bill S. Hansson. Olfactory receptor genes of the spruce bark beetle, Ips typographus (manuscript). II Martin N. Andersson, Mattias C. Larsson, Fredrik Schlyter (2009). Specificity and redundancy in the olfactory system of the bark beetle Ips typographus: Single-cell responses to ecologically relevant odors. Journal of Insect Physiology 55, 556-567. III Martin N. Andersson, Mattias C. Larsson, Miroslav Blaženec, Rastislav Jakuš, Qing-He Zhang, Fredrik Schlyter (2010). Peripheral modulation of pheromone response by inhibitory host compound in a beetle. Journal of Experimental Biology 213, 3332-3339. IV Martin N. Andersson, Fredrik Schlyter, Sharon Rose Hill, and Teun Dekker. What reaches the olfactory receptor? -Factors affecting vapor concentration and interpretation of chemosensory studies (manuscript). V Martin N. Andersson, Muhammad Binyameen, Medhat M. Sadek, Fredrik Schlyter. Attraction modulated by spacing of pheromone components and anti-attractants in a bark beetle and a moth (submitted).
Place of Publication:Alnarp, Sweden
ISBN for printed version:978-91-576-7573-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:ips typographus, sensory receptors, neurophysiology, ionization
Keywords:Ips typographus, odorant receptor, olfactory receptor neuron, electro¬physiology, co-localization, photoionization detector, odor spacing
URN:NBN:urn:nbn:se:slu:epsilon-3145
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3145
ID Code:2428
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: Martin Andersson
Deposited On:17 Jan 2011 00:00
Metadata Last Modified:02 Dec 2014 10:18

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