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Evidence for a synergistic effect of post-translational modifications and genomic composition of eEF-1 alpha on the adaptation of Phytophthora infestans

Wang, Yan-Ping and Wu, E-Jiao and Lurwanu, Yahuza and Ding, Ji-Peng and He, Dun-Chun and Waheed, Abdul and Nkurikiyimfura, Oswald and Liu, Shi-Ting and Li, Wen-Yang and Wang, Zong-Hua and Yang, Lina and Zhan, Jiasui (2021). Evidence for a synergistic effect of post-translational modifications and genomic composition of eEF-1 alpha on the adaptation of Phytophthora infestans. Ecology and Evolution. 11 , 5484-5496
[Research article]

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Abstract

Genetic variation plays a fundamental role in pathogen's adaptation to environmental stresses. Pathogens with low genetic variation tend to survive and proliferate more poorly due to their lack of genotypic/phenotypic polymorphisms in responding to fluctuating environments. Evolutionary theory hypothesizes that the adaptive disadvantage of genes with low genomic variation can be compensated for structural diversity of proteins through post-translation modification (PTM) but this theory is rarely tested experimentally and its implication to sustainable disease management is hardly discussed. In this study, we analyzed nucleotide characteristics of eukaryotic translation elongation factor-1 alpha (eEF-l alpha) gene from 165 Phytophthora infestans isolates and the physical and chemical properties of its derived proteins. We found a low sequence variation of eEF-l alpha protein, possibly attributable to purifying selection and a lack of intra-genic recombination rather than reduced mutation. In the only two isoforms detected by the study, the major one accounted for >95% of the pathogen collection and displayed a significantly higher fitness than the minor one. High lysine representation enhances the opportunity of the eEF-1 alpha protein to be methylated and the absence of disulfide bonds is consistent with the structural prediction showing that many disordered regions are existed in the protein. Methylation, structural disordering, and possibly other PTMs ensure the ability of the protein to modify its functions during biological, cellular and biochemical processes, and compensate for its adaptive disadvantage caused by sequence conservation. Our results indicate that PTMs may function synergistically with nucleotide codes to regulate the adaptive landscape of eEF-1 alpha, possibly as well as other housekeeping genes, in P. infestans. Compensatory evolution between pre- and post-translational phase in eEF-1 alpha could enable pathogens quickly adapting to disease management strategies while efficiently maintaining critical roles of the protein playing in biological, cellular, and biochemical activities. Implications of these results to sustainable plant disease management are discussed.

Authors/Creators:Wang, Yan-Ping and Wu, E-Jiao and Lurwanu, Yahuza and Ding, Ji-Peng and He, Dun-Chun and Waheed, Abdul and Nkurikiyimfura, Oswald and Liu, Shi-Ting and Li, Wen-Yang and Wang, Zong-Hua and Yang, Lina and Zhan, Jiasui
Title:Evidence for a synergistic effect of post-translational modifications and genomic composition of eEF-1 alpha on the adaptation of Phytophthora infestans
Series Name/Journal:Ecology and Evolution
Year of publishing :2021
Volume:11
Page range:5484-5496
Number of Pages:13
Publisher:WILEY
ISSN:2045-7758
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
Keywords:adaptation, compensatory evolution, housekeeping genes, methylation, plant pathogens, post‐translational modification, protein disordering, sustainable disease management
URN:NBN:urn:nbn:se:slu:epsilon-p-111397
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111397
Additional ID:
Type of IDID
DOI10.1002/ece3.7442
Web of Science (WoS)000630047800001
ID Code:24347
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:01 Jun 2021 14:43
Metadata Last Modified:01 Jun 2021 14:51

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