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Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender

Hermansson, Ulrika and Johannisson, Anders and Axner, Eva (2021). Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender. Veterinary Medicine and Science. 7 , 812-819
[Research article]

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Abstract

Egg yolk is widely used as a cryoprotectant in dog semen extenders, but there is a risk of contamination with animal pathogens. In addition, egg yolk may vary in composition, making it difficult to standardize the extender. Lecithin is an animal protein-free alternative to egg yolk for semen cryopreservation. Recently, it was shown that 1% of soybean lecithin type II-S was better than 2% for freezing canine semen. The aim of the study was to compare two different types of soybean lecithin, with egg yolk as a control. Ejaculates from eight dogs were divided into three equal parts and diluted with a Tris-based extender, containing either 20% egg yolk, 1% soybean lecithin Type II-S or 1% soybean lecithin Type IV-S. The samples were then frozen. Sperm motility was evaluated by computer-assisted sperm analysis (CASA), acrosome integrity (FITC-PNA/PI) and sperm membrane integrity (SYBR-14/PI) post-thaw, as well as after 2 and 4 hr incubation at 37 degrees C. Post-thaw sperm chromatin structure assay and plasma membrane integrity were evaluated by flow cytometry. Total motility, sperm plasma membrane integrity and acrosome integrity were significantly better in the egg yolk extender than in the two soybean lecithin-based extenders. Individual motility post-thaw differed more than in the fresh samples, illustrating individual differences in tolerance to the cryostress. The DNA Fragmentation Index (% DFI) was significantly lower in the Tris egg yolk (TEY) extender compared to any of the soybean-based extenders. The number of high green stained spermatozoa were significantly higher in Type IV-S compared to the control TEY extender. In conclusion, egg yolk was superior to the two lecithin-based extenders to cryopreserve canine semen.

Authors/Creators:Hermansson, Ulrika and Johannisson, Anders and Axner, Eva
Title:Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender
Series Name/Journal:Veterinary Medicine and Science
Year of publishing :2021
Volume:7
Page range:812-819
Number of Pages:8
Publisher:WILEY
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:canine, extender, reproduction, reproductive tract, semen freezing, soybean lecithin
URN:NBN:urn:nbn:se:slu:epsilon-p-110767
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110767
Additional ID:
Type of IDID
DOI10.1002/vms3.445
Web of Science (WoS)000616916500001
ID Code:24362
Faculty:University Animal Hospital
VH - Faculty of Veterinary Medicine and Animal Science
Department:University Animal Hospital (UAH)
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:02 Jun 2021 11:24
Metadata Last Modified:02 Jun 2021 11:31

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