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Loss of Arabidopsis matrix metalloproteinase-5 affects root development and root bacterial communities during drought stress

Mishra, Laxmi S. and Kim, Sung-Yong and Caddell, Daniel F. and Coleman-Derr, Devin and Funk, Christiane (2021). Loss of Arabidopsis matrix metalloproteinase-5 affects root development and root bacterial communities during drought stress. Physiologia Plantarum. 172 , 1045-1058
[Research article]

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Abstract

Matrix metalloproteinases (MMPs) are zinc-dependent endo-peptidases that in mammals are known to be involved in remodeling the extracellular matrix (ECM) in developmental and pathological processes. In this study, we report At5-MMP of Arabidopsis thaliana to be important for root development and root bacterial communities. At5-MMP is mainly localized in the root vasculature and lateral root, an At5-MMP T-DNA insertion mutant (mmp5 KO) showed reduced root growth and a lower number of root apexes, causing reduced water uptake from the soil. Subsequently, mmp5 KO is sensitive to drought stress. Inhibited auxin transport was accompanied with resistance to indole-3-acetic acid (IAA), 2, 4-dichlorophenoxyacetic acid (2, 4-D), and 1-naphthaleneacetic acid (NAA). The content of endogenous abscisic acid (ABA) was lower in roots of mmp5 KO than in wild type. Genes responsive to ABA as well as genes encoding enzymes of the proline biosynthesis were expressed to a lower extent in mmp5 KO than in wild type. Moreover, drought stress modulated root-associated bacterial communities of mmp5 KO: the number of Actinobacteria increased. Therefore, At5-MMP modulates auxin/ABA signaling rendering the plant sensitive to drought stress and recruiting differential root bacterial communities.

Authors/Creators:Mishra, Laxmi S. and Kim, Sung-Yong and Caddell, Daniel F. and Coleman-Derr, Devin and Funk, Christiane
Title:Loss of Arabidopsis matrix metalloproteinase-5 affects root development and root bacterial communities during drought stress
Series Name/Journal:Physiologia Plantarum
Year of publishing :2021
Volume:172
Page range:1045-1058
Number of Pages:14
Publisher:WILEY
Associated Programs and Other Stakeholders:?? SLU.id.platform-plantprot ??
ISSN:0031-9317
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
URN:NBN:urn:nbn:se:slu:epsilon-p-109505
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109505
Additional ID:
Type of IDID
DOI10.1111/ppl.13299
Web of Science (WoS)000599096900001
ID Code:24368
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:02 Jun 2021 12:01
Metadata Last Modified:02 Jun 2021 12:11

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