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Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis

Huang, Lei and Li, Xiaohui and Zhang, Weiwei and Ung, Nolan and Liu, Nana and Yin, Xianglin and Li, Yong and Mcewan, Robert E. and Dilkes, Brian and Dai, Mingji and Hicks, Glenn and Raikhel, Natasha V. and Staiger, Christopher J. and Zhang, Chunhua (2020). Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis. Plant Cell. 32 , 2141-2157
[Research article]

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Abstract

The cellulose synthase inhibitor Endosidin20 is an excellent tool for manipulating cellulose synthesis in order to produce cellulose products with desired properties and to control weeds. Plant cellulose is synthesized by rosette-structured cellulose synthase (CESA) complexes (CSCs). Each CSC is composed of multiple subunits of CESAs representing three different isoforms. Individual CESA proteins contain conserved catalytic domains for catalyzing cellulose synthesis, other domains such as plant-conserved sequences, and class-specific regions that are thought to facilitate complex assembly and CSC trafficking. Because of the current lack of atomic-resolution structures for plant CSCs or CESAs, the molecular mechanism through which CESA catalyzes cellulose synthesis and whether its catalytic activity influences efficient CSC transport at the subcellular level remain unknown. Here, by performing chemical genetic analyses, biochemical assays, structural modeling, and molecular docking, we demonstrate that Endosidin20 (ES20) targets the catalytic site of CESA6 in Arabidopsis (Arabidopsis thaliana). Chemical genetic analysis revealed important amino acids that potentially participate in the catalytic activity of plant CESA6, in addition to previously identified conserved motifs across kingdoms. Using high spatiotemporal resolution live cell imaging, we found that inhibiting the catalytic activity of CESA6 by ES20 treatment reduced the efficiency of CSC transport to the plasma membrane. Our results demonstrate that ES20 is a chemical inhibitor of CESA activity and trafficking that represents a powerful tool for studying cellulose synthesis in plants.

Authors/Creators:Huang, Lei and Li, Xiaohui and Zhang, Weiwei and Ung, Nolan and Liu, Nana and Yin, Xianglin and Li, Yong and Mcewan, Robert E. and Dilkes, Brian and Dai, Mingji and Hicks, Glenn and Raikhel, Natasha V. and Staiger, Christopher J. and Zhang, Chunhua
Title:Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis
Series Name/Journal:Plant Cell
Year of publishing :2020
Volume:32
Page range:2141-2157
Number of Pages:17
Publisher:AMER SOC PLANT BIOLOGISTS
Associated Programs and Other Stakeholders:?? SLU.id.platform-plantprot ??
ISSN:1040-4651
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-107011
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107011
Additional ID:
Type of IDID
DOI10.1105/tpc.20.00202
Web of Science (WoS)000545974100018
ID Code:24585
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:21 Jun 2021 14:46
Metadata Last Modified:21 Jun 2021 14:51

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