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Polymerase IV Plays a Crucial Role in Pollen Development in Capsella

Wang, Zhenxing and Butel, Nicolas and Santos-González, Juan and Borges, Filipe and Yi, Jun and Martienssen, Robert A. and Martinez Arias, German and Köhler, Claudia (2020). Polymerase IV Plays a Crucial Role in Pollen Development in Capsella. Plant Cell. 32 , 950-966
[Research article]

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Abstract

Unlike in Arabidopsis, loss of function of Polymerase IV in the Brassicaceae species Capsella rubella causes microspore arrest, revealing an important role for Polymerase IV in pollen development.In Arabidopsis (Arabidopsis thaliana), DNA-dependent RNA polymerase IV (Pol IV) is required for the formation of transposable element (TE)-derived small RNA transcripts. These transcripts are processed by DICER-LIKE3 into 24-nucleotide small interfering RNAs (siRNAs) that guide RNA-directed DNA methylation. In the pollen grain, Pol IV is also required for the accumulation of 21/22-nucleotide epigenetically activated siRNAs, which likely silence TEs via post-transcriptional mechanisms. Despite this proposed role of Pol IV, its loss of function in Arabidopsis does not cause a discernible pollen defect. Here, we show that the knockout of NRPD1, encoding the largest subunit of Pol IV, in the Brassicaceae species Capsella (Capsella rubella), caused postmeiotic arrest of pollen development at the microspore stage. As in Arabidopsis, all TE-derived siRNAs were depleted in Capsella nrpd1 microspores. In the wild-type background, the same TEs produced 21/22-nucleotide and 24-nucleotide siRNAs; these processes required Pol IV activity. Arrest of Capsella nrpd1 microspores was accompanied by the deregulation of genes targeted by Pol IV-dependent siRNAs. TEs were much closer to genes in Capsella compared with Arabidopsis, perhaps explaining the essential role of Pol IV in pollen development in Capsella. Our discovery that Pol IV is functionally required in Capsella microspores emphasizes the relevance of investigating different plant models.

Authors/Creators:Wang, Zhenxing and Butel, Nicolas and Santos-González, Juan and Borges, Filipe and Yi, Jun and Martienssen, Robert A. and Martinez Arias, German and Köhler, Claudia
Title:Polymerase IV Plays a Crucial Role in Pollen Development in Capsella
Series Name/Journal:Plant Cell
Year of publishing :2020
Volume:32
Page range:950-966
Number of Pages:17
Publisher:AMER SOC PLANT BIOLOGISTS
ISSN:1040-4651
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
URN:NBN:urn:nbn:se:slu:epsilon-p-105731
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105731
Additional ID:
Type of IDID
DOI10.1105/tpc.19.00938
Web of Science (WoS)000528827100028
ID Code:24645
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:21 Jun 2021 15:27
Metadata Last Modified:21 Jun 2021 15:34

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