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Virus Diversity and Loads in Crickets Reared for Feed: Implications for Husbandry

Rodrigues De Miranda, Joachim and Granberg, Fredrik and Low, Matthew and Onorati, Piero and Semberg, Emilia and Jansson, Anna and Berggren, Åsa (2021). Virus Diversity and Loads in Crickets Reared for Feed: Implications for Husbandry. Frontiers in Veterinary Science. 8 , 642085
[Research article]

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Abstract

Insects generally have high reproductive rates leading to rapid population growth and high local densities; ideal conditions for disease epidemics. The parasites and diseases that naturally regulate wild insect populations can also impact when these insects are produced commercially, on farms. While insects produced for human or animal consumption are often reared under high density conditions, very little is known about the microbes associated with these insects, particularly those with pathogenic potential. In this study we used both target-free and targeted screening approaches to explore the virome of two cricket species commonly reared for feed and food, Acheta domesticus and Gryllus bimaculatus. The target-free screening of DNA and RNA from a single A. domesticus frass sample revealed that only 1% of the nucleic acid reads belonged to viruses, including known cricket, insect, bacterial and plant pathogens, as well as a diverse selection of novel viruses. The targeted screening revealed relatively high levels of Acheta domesticus densovirus, invertebrate iridovirus 6 and a novel iflavirus, as well as low levels of Acheta domesticus volvovirus, in insect and frass samples from several retailers. Our findings highlight the value of multiple screening approaches for a comprehensive and robust cricket disease monitoring and management strategy. This will become particularly relevant as-and-when cricket rearing facilities scale up and transform from producing insects for animal feed to producing insects for human consumption.

Authors/Creators:Rodrigues De Miranda, Joachim and Granberg, Fredrik and Low, Matthew and Onorati, Piero and Semberg, Emilia and Jansson, Anna and Berggren, Åsa
Title:Virus Diversity and Loads in Crickets Reared for Feed: Implications for Husbandry
Series Name/Journal:Frontiers in Veterinary Science
Year of publishing :2021
Volume:8
Article number:642085
Number of Pages:10
Publisher:FRONTIERS MEDIA SA
ISSN:2297-1769
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:metagenome, virome, Acheta domesticus densovirus, invertebrate iridovirus 6, Acheta domesticus, cricket rearing, frass, Acheta domesticus iflavirus
URN:NBN:urn:nbn:se:slu:epsilon-p-112567
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112567
Additional ID:
Type of IDID
DOI10.3389/fvets.2021.642085
Web of Science (WoS)000657274700001
ID Code:24742
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology

(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:29 Jun 2021 09:26
Metadata Last Modified:29 Jun 2021 09:31

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