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The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis

Cseplo, Agnes and Zsigmond, Laura and Andrasi, Norbert and Baba, Abu and Labhane, Nitin M. and Peto, Andrea and Kovacs, Hajnalka E. and Steinbach, Gabor and Szabados, Laszlo and Feher, Attila and Rigo, Gabor (2021). The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis. International Journal of Molecular Sciences. 22 , 5979
[Research article]

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Abstract

The Arabidopsis AtCRK5 protein kinase is involved in the establishment of the proper auxin gradient in many developmental processes. Among others, the Atcrk5-1 mutant was reported to exhibit a delayed gravitropic response via compromised PIN2-mediated auxin transport at the root tip. Here, we report that this phenotype correlates with lower superoxide anion (O-2(center dot-)) and hydrogen peroxide (H2O2) levels but a higher nitric oxide (NO) content in the mutant root tips in comparison to the wild type (AtCol-0). The oxidative stress inducer paraquat (PQ) triggering formation of O-2(center dot-) (and consequently, H2O2) was able to rescue the gravitropic response of Atcrk5-1 roots. The direct application of H2O2 had the same effect. Under gravistimulation, correct auxin distribution was restored (at least partially) by PQ or H2O2 treatment in the mutant root tips. In agreement, the redistribution of the PIN2 auxin efflux carrier was similar in the gravistimulated PQ-treated mutant and untreated wild type roots. It was also found that PQ-treatment decreased the endogenous NO level at the root tip to normal levels. Furthermore, the mutant phenotype could be reverted by direct manipulation of the endogenous NO level using an NO scavenger (cPTIO). The potential involvement of AtCRK5 protein kinase in the control of auxin-ROS-NO-PIN2-auxin regulatory loop is discussed.

Authors/Creators:Cseplo, Agnes and Zsigmond, Laura and Andrasi, Norbert and Baba, Abu and Labhane, Nitin M. and Peto, Andrea and Kovacs, Hajnalka E. and Steinbach, Gabor and Szabados, Laszlo and Feher, Attila and Rigo, Gabor
Title:The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2021
Volume:22
Article number:5979
Number of Pages:23
Publisher:MDPI
ISSN:1661-6596
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:auxin transport, Calcium-Dependent Protein Kinase-Related Kinase (CRK), reactive oxygen species, superoxide anion, hydrogen peroxide, nitric oxide, paraquat, oxidative stress, root gravitropism, Arabidopsis
URN:NBN:urn:nbn:se:slu:epsilon-p-112609
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112609
Additional ID:
Type of IDID
DOI10.3390/ijms22115979
Web of Science (WoS)000660147500001
ID Code:24759
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:30 Jun 2021 08:25
Metadata Last Modified:30 Jun 2021 08:31

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