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Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate

Brandenburg, Jule and Blomqvist, Johanna and Shapaval, Volha and Kohler, Achim and Sampels, Sabine and Sandgren, Mats and Passoth, Volkmar (2021). Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate. Biotechnology for Biofuels. 14 , 124
[Research article]

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Abstract

Background Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation physiology in different oleaginous yeasts grown on the various carbon sources present in lignocellulose hydrolysate (LH), new targets for optimisation of lipid accumulation can be identified. Monitoring lipid formation over time is essential for understanding lipid accumulation physiology. This study investigated lipid accumulation in a variety of oleaginous ascomycetous and basidiomycetous strains grown in glucose and xylose and followed lipid formation kinetics of selected strains in wheat straw hydrolysate (WSH). Results Twenty-nine oleaginous yeast strains were tested for their ability to utilise glucose and xylose, the main sugars present in WSH. Evaluation of sugar consumption and lipid accumulation revealed marked differences in xylose utilisation capacity between the yeast strains, even between those belonging to the same species. Five different promising strains, belonging to the species Lipomyces starkeyi, Rhodotorula glutinis, Rhodotorula babjevae and Rhodotorula toruloides, were grown on undiluted wheat straw hydrolysate and lipid accumulation was followed over time, using Fourier transform-infrared (FTIR) spectroscopy. All five strains were able to grow on undiluted WSH and to accumulate lipids, but to different extents and with different productivities. R. babjevae DVBPG 8058 was the best-performing strain, accumulating 64.8% of cell dry weight (CDW) as lipids. It reached a culture density of 28 g/L CDW in batch cultivation, resulting in a lipid content of 18.1 g/L and yield of 0.24 g lipids per g carbon source. This strain formed lipids from the major carbon sources in hydrolysate, glucose, acetate and xylose. R. glutinis CBS 2367 also consumed these carbon sources, but when assimilating xylose it consumed intracellular lipids simultaneously. Rhodotorula strains contained a higher proportion of polyunsaturated fatty acids than the two tested Lipomyces starkeyi strains. Conclusions There is considerable metabolic diversity among oleaginous yeasts, even between closely related species and strains, especially when converting xylose to biomass and lipids. Monitoring the kinetics of lipid accumulation and identifying the molecular basis of this diversity are keys to selecting suitable strains for high lipid production from lignocellulose.

Authors/Creators:Brandenburg, Jule and Blomqvist, Johanna and Shapaval, Volha and Kohler, Achim and Sampels, Sabine and Sandgren, Mats and Passoth, Volkmar
Title:Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate
Series Name/Journal:Biotechnology for Biofuels
Year of publishing :2021
Volume:14
Article number:124
Number of Pages:12
Publisher:BMC
ISSN:1754-6834
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 207 Environmental Engineering > Energy Systems
Keywords:Oleaginous yeasts, Ascomycetes, Basidiomycetes, FTIR, Lipids, Lignocellulose, Xylose, Biofuels
URN:NBN:urn:nbn:se:slu:epsilon-p-112570
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112570
Additional ID:
Type of IDID
DOI10.1186/s13068-021-01974-2
Web of Science (WoS)000659100100002
ID Code:24770
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:30 Jun 2021 13:25
Metadata Last Modified:30 Jun 2021 13:31

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