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Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells

Chankeaw, Wiruntita and Lignier, Sandra and Richard, Christophe and Ntallaris, Theodoros and Raliou, Mariam and Guo, Yongzhi and Plassard, Damien and Bevilacqua, Claudia and Sandra, Olivier and Andersson, Göran and Humblot, Patrice and Charpigny, Gilles (2021). Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells. BMC Genomics. 22 , 450
[Research article]

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Abstract

Background In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB. Results Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE. Conclusion The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.

Authors/Creators:Chankeaw, Wiruntita and Lignier, Sandra and Richard, Christophe and Ntallaris, Theodoros and Raliou, Mariam and Guo, Yongzhi and Plassard, Damien and Bevilacqua, Claudia and Sandra, Olivier and Andersson, Göran and Humblot, Patrice and Charpigny, Gilles
Title:Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells
Series Name/Journal:BMC Genomics
Year of publishing :2021
Volume:22
Article number:450
Number of Pages:18
Publisher:BMC
ISSN:1471-2164
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Negative energy balance, Endometrium, Cell type-specific, Transcriptome, LCM, RNA-seq, Inflammation
URN:NBN:urn:nbn:se:slu:epsilon-p-112745
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112745
Additional ID:
Type of IDID
DOI10.1186/s12864-021-07713-z
Web of Science (WoS)000664630000002
ID Code:24839
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:12 Jul 2021 07:25
Metadata Last Modified:12 Jul 2021 07:31

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