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Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers

Morris, Ellen Ruth A. and Boyle, Ashley G. and Riihimäki, Miia and Aspán, Anna and Anis, Eman and Hillhouse, Andrew E. and Ivanov, Ivan and Bordin, Angela I. and Pringle, John and Cohen, Noah D. (2021). Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers. PLoS ONE. 16 , e0252804
[Research article]

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Abstract

Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses because it neither persists long-term in the environment nor infects other host mammals or vectors, and infection results in short-lived immunity. Whether pathogen factors enable SEE to remain in horses without causing clinical signs remains poorly understood. Thus, our objective was to use next-generation sequencing technologies to characterize the genome, methylome, and transcriptome of isolates of SEE from horses with acute clinical strangles and inapparent carrier horses-including isolates recovered from individual horses sampled repeatedly-to assess pathogen-associated changes that might reflect specific adaptions of SEE to the host that contribute to inapparent carriage. The accessory genome elements and methylome of SEE isolates from Sweden and Pennsylvania revealed no significant or consistent differences between acute clinical and inapparent carrier isolates of SEE. RNA sequencing of SEE isolates from Pennsylvania demonstrated no genes that were differentially expressed between acute clinical and inapparent carrier isolates of SEE. The absence of specific, consistent changes in the accessory genomes, methylomes, and transcriptomes of acute clinical and inapparent carrier isolates of SEE indicates that adaptations of SEE to the host are unlikely to explain the carrier state of SEE. Efforts to understand the carrier state of SEE should instead focus on host factors.

Authors/Creators:Morris, Ellen Ruth A. and Boyle, Ashley G. and Riihimäki, Miia and Aspán, Anna and Anis, Eman and Hillhouse, Andrew E. and Ivanov, Ivan and Bordin, Angela I. and Pringle, John and Cohen, Noah D.
Title:Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers
Series Name/Journal:PLoS ONE
Year of publishing :2021
Volume:16
Article number:e0252804
Number of Pages:19
Publisher:PUBLIC LIBRARY SCIENCE
ISSN:1932-6203
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
URN:NBN:urn:nbn:se:slu:epsilon-p-112761
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112761
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0252804
Web of Science (WoS)000664643500016
ID Code:24843
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:12 Jul 2021 11:25
Metadata Last Modified:12 Jul 2021 11:31

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