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Serological Evidence of Multiple Zoonotic Viral Infections among Wild Rodents in Barbados

Cayol, Claire and Forbes, Kristian Michael and Vapalahti, Olli and Niemi, Henrik and Sironen, Tarja (2021). Serological Evidence of Multiple Zoonotic Viral Infections among Wild Rodents in Barbados. Pathogens. 10 , 663
[Research article]

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Abstract

Background: Rodents are reservoirs for several zoonotic pathogens that can cause human infectious diseases, including orthohantaviruses, mammarenaviruses and orthopoxviruses. Evidence exists for these viruses circulating among rodents and causing human infections in the Americas, but much less evidence exists for their presence in wild rodents in the Caribbean. Methods: Here, we conducted serological and molecular investigations of wild rodents in Barbados to determine the prevalence of orthohantavirus, mammarenavirus and orthopoxvirus infections, and the possible role of these rodent species as reservoirs of zoonotic pathogens. Using immunofluorescent assays (IFA), rodent sera were screened for the presence of antibodies to orthohantavirus, mammarenavirus (Lymphocytic choriomeningitis virus-LCMV) and orthopoxvirus (Cowpox virus-CPXV) infections. RT-PCR was then conducted on orthohantavirus and mammarenavirus-seropositive rodent sera and tissues, to detect the presence of viral RNA. Results: We identified antibodies against orthohantavirus, mammarenavirus, and orthopoxvirus among wild mice and rats (3.8%, 2.5% and 7.5% seropositivity rates respectively) in Barbados. No orthohantavirus or mammarenavirus viral RNA was detected from seropositive rodent sera or tissues using RT-PCR. Conclusions: Key findings of this study are the first serological evidence of orthohantavirus infections in Mus musculus and the first serological evidence of mammarenavirus and orthopoxvirus infections in Rattus norvegicus and M. musculus in the English-speaking Caribbean. Rodents may present a potential zoonotic and biosecurity risk for transmission of three human pathogens, namely orthohantaviruses, mammarenaviruses and orthopoxviruses in Barbados.

Authors/Creators:Cayol, Claire and Forbes, Kristian Michael and Vapalahti, Olli and Niemi, Henrik and Sironen, Tarja
Title:Serological Evidence of Multiple Zoonotic Viral Infections among Wild Rodents in Barbados
Series Name/Journal:Pathogens
Year of publishing :2021
Volume:10
Article number:663
Number of Pages:14
Publisher:MDPI
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Keywords:orthohantavirus, infectious disease, Caribbean, zoonosis, biosecurity
URN:NBN:urn:nbn:se:slu:epsilon-p-112768
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112768
Additional ID:
Type of IDID
DOI10.3390/pathogens10060663
Web of Science (WoS)000666710900001
ID Code:24850
Faculty:Library
Department:Common Units > SLU University Library
Deposited By: SLUpub Connector
Deposited On:13 Jul 2021 09:25
Metadata Last Modified:13 Jul 2021 09:31

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