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Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations

Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Tsuji, Jackson M. and Neufeld, Josh D. and McMahon, Katherine D. and Bertilsson, Stefan and Greening, Christian and Peura, Sari (2021). Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations. mSystems. 6 , e01196-20
[Research article]

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Abstract

Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems.

Authors/Creators:Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Tsuji, Jackson M. and Neufeld, Josh D. and McMahon, Katherine D. and Bertilsson, Stefan and Greening, Christian and Peura, Sari
Title:Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations
Series Name/Journal:mSystems
Year of publishing :2021
Volume:6
Article number:e01196-20
ISSN:2379-5077
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Chlorobia, freshwater, photosynthetic bacteria, planktonic
URN:NBN:urn:nbn:se:slu:epsilon-p-112839
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112839
Additional ID:
Type of IDID
DOI10.1128/mSystems.01196-20
ID Code:24885
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:12 Aug 2021 14:09
Metadata Last Modified:12 Aug 2021 14:11

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