Home About Browse Search
Svenska


Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs

Hammer, S. E. and Duckova, T. and Groiss, S. and Stadler, M. and Jensen-Waern, Marianne and Golde, W. T. and Gimsa, U. and Saalmueller, A. (2021). Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs. Animal Genetics. 52 , 523-531
[Research article]

[img] PDF
674kB

Abstract

In Europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments and their use in infection studies and for design of veterinary vaccines. In this study, we characterised the SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes of 549 farmed pigs representing nine commercial pig lines by low-resolution (Lr) SLA haplotyping. In total, 50 class I and 37 class II haplotypes were identified in the studied cohort. The most common SLA class I haplotypes Lr-04.0 (SLA-1*04XX-SLA-3*04XX(04:04)-SLA-2*04XX) and Lr-32.0 (SLA-1*07XX-SLA-3*04XX(04:04)-SLA-2*02XX) occurred at frequencies of 11.02 and 8.20% respectively. For SLA class II, the most prevalent haplotypes Lr-0.15b (DRB1*04XX(04:05/04:06)-DQB1*02XX(02:02)-DQA*02XX) and Lr-0.12 (DRB1*06XX-DQB1*07XX-DQA*01XX) occurred at frequencies of 14.37 and 12.46% respectively. Meanwhile, our laboratory has contributed to several vaccine correlation studies (e.g. Porcine Reproductive and Respiratory Syndrome Virus, Classical Swine Fever Virus, Foot-and-Mouth Disease Virus and Swine Influenza A Virus) elucidating the immunodominance in the T-cell response with antigen specificity dependent on certain SLA-I and SLA-II haplotypes. Moreover, these SLA-immune response correlations could facilitate tailored vaccine development, as SLA-I Lr-04.0 and Lr-32.0 as well as SLA-II Lr-0.15b and Lr-0.12 are highly abundant haplotypes in European farmed pigs.

Authors/Creators:Hammer, S. E. and Duckova, T. and Groiss, S. and Stadler, M. and Jensen-Waern, Marianne and Golde, W. T. and Gimsa, U. and Saalmueller, A.
Title:Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs
Series Name/Journal:Animal Genetics
Year of publishing :2021
Volume:52
Page range:523-531
Number of Pages:9
Publisher:WILEY
ISSN:0268-9146
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:polymorphism, sequence‐specific primers PCR, Sus scrofa, swine leukocyte antigen
URN:NBN:urn:nbn:se:slu:epsilon-p-112098
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112098
Additional ID:
Type of IDID
DOI10.1111/age.13090
Web of Science (WoS)000653251900001
ID Code:24982
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:23 Aug 2021 10:25
Metadata Last Modified:23 Aug 2021 10:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits