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Enhanced detection of ATTR amyloid using a nanofibril-based assay

Rahman, Mahafuzur and Schmuck, Benjamin and Hansson, Henrik and Härd, Torleif and Westermark, Gunilla T. and Sandgren, Mats (2021). Enhanced detection of ATTR amyloid using a nanofibril-based assay. Amyloid. 28 , 158-167
[Research article]

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Abstract

More than 30 proteins and peptides have been found to form amyloid fibrils in human diseases. Fibrils formed by transthyretin (TTR) are associated with ATTR amyloidosis, affecting many vital organs, including the heart and peripheral nervous system. Congo red staining is the gold standard method for detection of amyloid deposits in tissue. However, Congo red staining and amyloid typing methods such as immunofluorescence labelling are limited to relatively large deposits. Detection of small ATTR deposits present at an early stage of the disease could enable timely treatment and prevent severe tissue damage. In this study, we developed an enhanced ATTR amyloid detection method that uses functionalised protein nanofibrils. Using this method, we achieved sensitive detection of monomeric TTR in a microplate immunoassay and immunofluorescence labelling of ex vivo tissue from two patients containing ATTR aggregates. The system's utility was confirmed on sections from a patient with AA amyloidosis and liver sections from inflamed mouse. These results suggest that the detection system constitutes important new technology for highly sensitive detection of microscopic amounts of ATTR amyloid deposited in tissue.

Authors/Creators:Rahman, Mahafuzur and Schmuck, Benjamin and Hansson, Henrik and Härd, Torleif and Westermark, Gunilla T. and Sandgren, Mats
Title:Enhanced detection of ATTR amyloid using a nanofibril-based assay
Series Name/Journal:Amyloid
Year of publishing :2021
Volume:28
Page range:158-167
Number of Pages:10
Publisher:TAYLOR AND FRANCIS LTD
ISSN:1350-6129
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 304 Medical Biotechnology > Biomedical Laboratory Science/Technology
Keywords:ATTR amyloid, AA amyloid, enhanced detection, functional protein nanofibril, immunoassay
URN:NBN:urn:nbn:se:slu:epsilon-p-110983
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110983
Additional ID:
Type of IDID
DOI10.1080/13506129.2021.1886072
Web of Science (WoS)000618006200001
ID Code:25013
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:24 Aug 2021 17:25
Metadata Last Modified:25 Aug 2021 06:57

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