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Larval response to frass and guaiacol: detection of an attractant produced by bacteria from Spodoptera littoralis frass

Revadi, Santosh and Giannuzzi, Vito Antonio and Vetukuri, Ramesh and Walker, William B. and Becher, Paul (2021). Larval response to frass and guaiacol: detection of an attractant produced by bacteria from Spodoptera littoralis frass. Journal of Pest Science. 94 , 1105-1118
[Research article]

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Abstract

Larval frass in herbivorous lepidopterans is mainly composed of plant-derived material and microbes from the gut. Despite the fact that frass from conspecific larvae repels female moths in Spodoptera littoralis from oviposition, the role of frass volatiles on larval foraging behavior is largely unknown. Here, we show that larvae of S. littoralis walk upwind to larval frass volatiles in a wind tunnel assay. We identified the frass volatile guaiacol (2-methoxyphenol) as key ligand for the S. littoralis odorant receptor (OR) SlitOr59 which we expressed heterologously. We isolated guaiacol-producing bacteria identified as Serratia marcescens from frass of larvae that were fed on cotton, and Enterobacter cloacae, E. ludwigii and Klebsiella sp. from frass derived from cabbage-fed larvae. In addition to guaiacol, we also identified volatiles acetoin, 3-methyl-1-butanol and dimethyl disulfide, in large proportions in headspace collections from the bacteria. A Y-tube olfactometer assay showed that fourth instar S. littoralis larvae are attracted to guaiacol. Moreover, cotton leaves treated with the insecticide Spinosad and guaiacol were highly attractive to the larvae. Our results provide a basis for management of the pest by directly targeting larvae, based on an attract-and-kill strategy. Further studies are needed to test the application of guaiacol for semiochemical-based pest management of Spodoptera pest species.

Authors/Creators:Revadi, Santosh and Giannuzzi, Vito Antonio and Vetukuri, Ramesh and Walker, William B. and Becher, Paul
Title:Larval response to frass and guaiacol: detection of an attractant produced by bacteria from Spodoptera littoralis frass
Series Name/Journal:Journal of Pest Science
Year of publishing :2021
Volume:94
Page range:1105-1118
Number of Pages:14
Publisher:SPRINGER HEIDELBERG
ISSN:1612-4758
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Bacteria, Caterpillar, Electrophysiology, Frass volatiles, Lepidoptera, Migration, Odorant receptor, Olfactometer
URN:NBN:urn:nbn:se:slu:epsilon-p-111408
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111408
Additional ID:
Type of IDID
DOI10.1007/s10340-021-01352-9
Web of Science (WoS)000625582700001
ID Code:25020
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:25 Aug 2021 13:25
Metadata Last Modified:25 Aug 2021 13:31

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