Revadi, Santosh and Giannuzzi, Vito Antonio and Vetukuri, Ramesh and Walker, William B. and Becher, Paul
(2021).
Larval response to frass and guaiacol: detection of an attractant produced by bacteria from Spodoptera littoralis frass.
Journal of Pest Science. 94
, 1105-1118
[Research article]
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Abstract
Larval frass in herbivorous lepidopterans is mainly composed of plant-derived material and microbes from the gut. Despite the fact that frass from conspecific larvae repels female moths in Spodoptera littoralis from oviposition, the role of frass volatiles on larval foraging behavior is largely unknown. Here, we show that larvae of S. littoralis walk upwind to larval frass volatiles in a wind tunnel assay. We identified the frass volatile guaiacol (2-methoxyphenol) as key ligand for the S. littoralis odorant receptor (OR) SlitOr59 which we expressed heterologously. We isolated guaiacol-producing bacteria identified as Serratia marcescens from frass of larvae that were fed on cotton, and Enterobacter cloacae, E. ludwigii and Klebsiella sp. from frass derived from cabbage-fed larvae. In addition to guaiacol, we also identified volatiles acetoin, 3-methyl-1-butanol and dimethyl disulfide, in large proportions in headspace collections from the bacteria. A Y-tube olfactometer assay showed that fourth instar S. littoralis larvae are attracted to guaiacol. Moreover, cotton leaves treated with the insecticide Spinosad and guaiacol were highly attractive to the larvae. Our results provide a basis for management of the pest by directly targeting larvae, based on an attract-and-kill strategy. Further studies are needed to test the application of guaiacol for semiochemical-based pest management of Spodoptera pest species.
Authors/Creators: | Revadi, Santosh and Giannuzzi, Vito Antonio and Vetukuri, Ramesh and Walker, William B. and Becher, Paul | ||||||
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Title: | Larval response to frass and guaiacol: detection of an attractant produced by bacteria from Spodoptera littoralis frass | ||||||
Series Name/Journal: | Journal of Pest Science | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 94 | ||||||
Page range: | 1105-1118 | ||||||
Number of Pages: | 14 | ||||||
Publisher: | SPRINGER HEIDELBERG | ||||||
ISSN: | 1612-4758 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||||
Keywords: | Bacteria, Caterpillar, Electrophysiology, Frass volatiles, Lepidoptera, Migration, Odorant receptor, Olfactometer | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-111408 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111408 | ||||||
Additional ID: |
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ID Code: | 25020 | ||||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||
Department: | (LTJ, LTV) > Department of Plant Protection Biology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 25 Aug 2021 13:25 | ||||||
Metadata Last Modified: | 25 Aug 2021 13:31 |
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