Furneaux, Brendan and Bahram, Mohammad and Rosling, Anna and Yorou, Nourou S. and Ryberg, Martin
(2021).
Long- and short-read metabarcoding technologies reveal similar spatiotemporal structures in fungal communities.
Molecular Ecology Resources. 21
, 1833-1849
[Research article]
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Abstract
Fungi form diverse communities and play essential roles in many terrestrial ecosystems, yet there are methodological challenges in taxonomic and phylogenetic placement of fungi from environmental sequences. To address such challenges, we investigated spatiotemporal structure of a fungal community using soil metabarcoding with four different sequencing strategies: short-amplicon sequencing of the ITS2 region (300-400 bp) with Illumina MiSeq, Ion Torrent Ion S5 and PacBio RS II, all from the same PCR library, as well as long-amplicon sequencing of the full ITS and partial LSU regions (1200-1600 bp) with PacBio RS II. Resulting community structure and diversity depended more on statistical method than sequencing technology. The use of long-amplicon sequencing enables construction of a phylogenetic tree from metabarcoding reads, which facilitates taxonomic identification of sequences. However, long reads present issues for denoising algorithms in diverse communities. We present a solution that splits the reads into shorter homologous regions prior to denoising, and then reconstructs the full denoised reads. In the choice between short and long amplicons, we suggest a hybrid approach using short amplicons for sampling breadth and depth, and long amplicons to characterize the local species pool for improved identification and phylogenetic analyses.
Authors/Creators: | Furneaux, Brendan and Bahram, Mohammad and Rosling, Anna and Yorou, Nourou S. and Ryberg, Martin | ||||||
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Title: | Long- and short-read metabarcoding technologies reveal similar spatiotemporal structures in fungal communities | ||||||
Series Name/Journal: | Molecular Ecology Resources | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 21 | ||||||
Page range: | 1833-1849 | ||||||
Number of Pages: | 17 | ||||||
Publisher: | WILEY | ||||||
ISSN: | 1755-098X | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-112012 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112012 | ||||||
Additional ID: |
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ID Code: | 25023 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Dept. of Ecology (S) > Dept. of Ecology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 25 Aug 2021 13:25 | ||||||
Metadata Last Modified: | 25 Aug 2021 13:31 |
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