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New methods for improving water management - Exploring the role of diatoms in ecosystems

Bailet, Bonnie (2021). New methods for improving water management - Exploring the role of diatoms in ecosystems. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880
ISBN 978-91-7760-788-5
eISBN 978-91-7760-789-2
[Doctoral thesis]

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Abstract

Diatoms are photosynthetic microalgae which are well known to react quickly to environmental changes and are widely used as bioindicators within Water Framework Directive. However, diatom identification by light microscopy is time-consuming and error-prone, creating a need for alternative, faster and more reproducible methods for characterising diatom communities. This thesis describes the development and application of two such methods: a molecular approach using metabarcoding and a trait-based approach using combinations of different diatom morphological traits. Both approaches were shown to have potential for use in environmental assessment, as they revealed the response of diatoms to environmental change and enabled better representation of the diatom community. However, results from the two approaches were not directly comparable to results obtained by conventional microscopy and diatom DNA and trait data should not be used with tools calibrated for morphotaxa data. Discrepancies in molecular output data between laboratories revealed a strong need for standardisation and best-practice guidelines. Nutrient indices developed for each novel approach performed well and showed potential for use in assessment of total phosphorus levels in Fennoscandia freshwater. The molecular-based index showed differences in performance for stream and lake samples. The trait-based index performed equally well for both types of site, but current limitation of this index is that selection of meaningful traits must be done for each new gradient. Further work is needed on diatom traits and their response to environmental changes, to obtain reproducible and comparable molecular outputs and to continue the development of new indices calibrated to diatom DNA and trait data.

Authors/Creators:Bailet, Bonnie
Title:New methods for improving water management - Exploring the role of diatoms in ecosystems
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2021
Number:2021:55
Number of Pages:113
Publisher:Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-788-5
ISBN for electronic version:978-91-7760-789-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Oceanography, Hydrology, Water Resources
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:ecological status, eutrophication, Bacillariophyta, morphological identification, 18S-V4, rbcL, ISU, ASV, trait classification, ecological guilds
URN:NBN:urn:nbn:se:slu:epsilon-p-113051
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113051
ID Code:25042
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:26 Aug 2021 07:25
Metadata Last Modified:26 Aug 2021 13:51

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