Ahi, Ehsan Pashay and Tsakoumis, Emmanouil and Brunel, Mathilde and Schmitz, Monika
(2021).
Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain.
Fish Physiology and Biochemistry. 47
, 1283-1298
[Research article]
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Abstract
The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. In this study, we used a loss of function leptin receptor zebrafish mutant and real-time quantitative PCR to assess brain expression patterns of several previously identified anorexigenic genes downstream of leptin signal under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-h refeeding). These downstream factors include members of cart genes, crhb and gnrh2, as well as selected genes co-expressed with them based on a zebrafish co-expression database. Here, we found a potential gene expression network (GRN) comprising the abovementioned genes by a stepwise approach of identifying co-expression modules and predicting their upstream regulators. Among the transcription factors (TFs) predicted as potential upstream regulators of this GRN, we found expression pattern of sp3a to be correlated with transcriptional changes of the downstream gene network. Interestingly, the expression and transcriptional activity of Sp3 orthologous gene in mammals have already been implicated to be under the influence of leptin signal. These findings suggest a potentially conserved regulatory connection between leptin and sp3a, which is predicted to act as a transcriptional driver of a downstream gene network in the zebrafish brain.
Authors/Creators: | Ahi, Ehsan Pashay and Tsakoumis, Emmanouil and Brunel, Mathilde and Schmitz, Monika | ||||||
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Title: | Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain | ||||||
Series Name/Journal: | Fish Physiology and Biochemistry | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 47 | ||||||
Page range: | 1283-1298 | ||||||
Number of Pages: | 16 | ||||||
Publisher: | SPRINGER | ||||||
ISSN: | 0920-1742 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402) (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science | ||||||
Keywords: | Leptin receptor, Zebrafish, Gene expression, Gene regulatory network, Feeding, Brain | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-113093 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113093 | ||||||
Additional ID: |
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ID Code: | 25051 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Department of Molecular Sciences | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 26 Aug 2021 11:25 | ||||||
Metadata Last Modified: | 26 Aug 2021 11:31 |
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