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Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain

Ahi, Ehsan Pashay and Tsakoumis, Emmanouil and Brunel, Mathilde and Schmitz, Monika (2021). Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain. Fish Physiology and Biochemistry. 47 , 1283-1298
[Research article]

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Abstract

The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. In this study, we used a loss of function leptin receptor zebrafish mutant and real-time quantitative PCR to assess brain expression patterns of several previously identified anorexigenic genes downstream of leptin signal under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-h refeeding). These downstream factors include members of cart genes, crhb and gnrh2, as well as selected genes co-expressed with them based on a zebrafish co-expression database. Here, we found a potential gene expression network (GRN) comprising the abovementioned genes by a stepwise approach of identifying co-expression modules and predicting their upstream regulators. Among the transcription factors (TFs) predicted as potential upstream regulators of this GRN, we found expression pattern of sp3a to be correlated with transcriptional changes of the downstream gene network. Interestingly, the expression and transcriptional activity of Sp3 orthologous gene in mammals have already been implicated to be under the influence of leptin signal. These findings suggest a potentially conserved regulatory connection between leptin and sp3a, which is predicted to act as a transcriptional driver of a downstream gene network in the zebrafish brain.

Authors/Creators:Ahi, Ehsan Pashay and Tsakoumis, Emmanouil and Brunel, Mathilde and Schmitz, Monika
Title:Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain
Series Name/Journal:Fish Physiology and Biochemistry
Year of publishing :2021
Volume:47
Page range:1283-1298
Number of Pages:16
Publisher:SPRINGER
ISSN:0920-1742
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
Keywords:Leptin receptor, Zebrafish, Gene expression, Gene regulatory network, Feeding, Brain
URN:NBN:urn:nbn:se:slu:epsilon-p-113093
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113093
Additional ID:
Type of IDID
DOI10.1007/s10695-021-00967-0
Web of Science (WoS)000670859300001
ID Code:25051
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:26 Aug 2021 11:25
Metadata Last Modified:26 Aug 2021 11:31

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