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Functional differences between TSHR alleles associate with variation in spawning season in Atlantic herring

Chen, Junfeng and Bi, Huijuan and Pettersson, Mats E. and Sato, Daiki X. and Fuentes-Pardo, Angela P. and Mo, Chunheng and Younis, Shady and Wallerman, Ola and Jern, Patric and Moles, Gregorio and Gomez, Ana and Kleinau, Gunnar and Scheerer, Patrick and Andersson, Leif (2021). Functional differences between TSHR alleles associate with variation in spawning season in Atlantic herring. Communications biology. 4 , 795
[Research article]

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Abstract

The underlying molecular mechanisms that determine long day versus short day breeders remain unknown in any organism. Atlantic herring provides a unique opportunity to examine the molecular mechanisms involved in reproduction timing, because both spring and autumn spawners exist within the same species. Although our previous whole genome comparisons revealed a strong association of TSHR alleles with spawning seasons, the functional consequences of these variants remain unknown. Here we examined the functional significance of six candidate TSHR mutations strongly associated with herring reproductive seasonality. We show that the L471M missense mutation in the spring-allele causes enhanced cAMP signaling. The best candidate non-coding mutation is a 5.2kb retrotransposon insertion upstream of the TSHR transcription start site, near an open chromatin region, which is likely to affect TSHR expression. The insertion occurred prior to the split between Pacific and Atlantic herring and was lost in the autumn-allele. Our study shows that strongly associated coding and non-coding variants at the TSHR locus may both contribute to the regulation of seasonal reproduction in herring. Junfeng Chen et al. examine potential functional consequences of reproduction timing-associated TSHR alleles segregating in Atlantic herring. By comparing fish that spawn during the spring to those that spawn in the autumn, they find that the spring-allele is correlated with enhanced cAMP signaling and that both coding and non-coding variants in the TSHR locus contribute to seasonal reproduction.

Authors/Creators:Chen, Junfeng and Bi, Huijuan and Pettersson, Mats E. and Sato, Daiki X. and Fuentes-Pardo, Angela P. and Mo, Chunheng and Younis, Shady and Wallerman, Ola and Jern, Patric and Moles, Gregorio and Gomez, Ana and Kleinau, Gunnar and Scheerer, Patrick and Andersson, Leif
Title:Functional differences between TSHR alleles associate with variation in spawning season in Atlantic herring
Series Name/Journal:Communications biology
Year of publishing :2021
Volume:4
Article number:795
Number of Pages:13
Publisher:NATURE RESEARCH
ISSN:2399-3642
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
URN:NBN:urn:nbn:se:slu:epsilon-p-113103
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113103
Additional ID:
Type of IDID
DOI10.1038/s42003-021-02307-7
Web of Science (WoS)000668739900001
ID Code:25064
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:26 Aug 2021 15:25
Metadata Last Modified:26 Aug 2021 15:31

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