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Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases

Muraleedharan, Madhu Nair and Karnaouri, Anthi and Piatkova, Maria and Ruiz-Caldas, Maria-Ximena and Matsakas, Leonidas and Liu, Bing and Rova, Ulrika and Christakopoulos, Paul and Mathew, Aji P. (2021). Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases. International Journal of Biological Macromolecules. 183 , 101-109
[Research article]

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Abstract

Nanocellulose isolation fromlignocellulose is a tedious and expensive processwith high energy and harsh chemical requirements, primarily due to the recalcitrance of the substrate, which otherwise would have been costeffective due to its abundance. Replacing the chemical steps with biocatalytic processes offers opportunities to solve this bottleneck to a certain extent due to the enzymes substrate specificity and mild reaction chemistry. In this work, we demonstrate the isolation of sulphate-free nanocellulose from organosolv pretreated birch biomass using different glycosyl-hydrolases, along with accessory oxidative enzymes including a lytic polysaccharide monooxygenase (LPMO). The suggested process produced colloidal nanocellulose suspensions (zeta-potential-19.4 mV) with particles of 7-20 nm diameter, high carboxylate content and improved thermostability (T-o= 301 degrees C, T-max= 337 degrees C). Nanocelluloseswere subjected to post-modification using LPMOs of different regioselectivity. The sample from chemical route was the least favorable for LPMO to enhance the carboxylate content, while that from the C1-specific LPMO treatment showed the highest increase in carboxylate content. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

Authors/Creators:Muraleedharan, Madhu Nair and Karnaouri, Anthi and Piatkova, Maria and Ruiz-Caldas, Maria-Ximena and Matsakas, Leonidas and Liu, Bing and Rova, Ulrika and Christakopoulos, Paul and Mathew, Aji P.
Title:Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases
Series Name/Journal:International Journal of Biological Macromolecules
Year of publishing :2021
Volume:183
Page range:101-109
Number of Pages:9
Publisher:ELSEVIER
ISSN:0141-8130
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 210 Nano Technology > Nano Technology
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Biocatalysis and Enzyme Technology
Keywords:Nanocellulose, LPMO biocatalysis, Post-treatment modification/functionalization
URN:NBN:urn:nbn:se:slu:epsilon-p-113219
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113219
Additional ID:
Type of IDID
DOI10.1016/j.ijbiomac.2021.04.136
Web of Science (WoS)000674774100010
ID Code:25117
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:30 Aug 2021 21:25
Metadata Last Modified:31 Aug 2021 05:01

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