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Fast protein liquid chromatography profiles of seminal plasma proteins in young bulls: A biomarker of sperm maturity?

Deori, Sourabh and Hurri, Emma and Karkehabadi, Saeid and Morrell, Jane (2021). Fast protein liquid chromatography profiles of seminal plasma proteins in young bulls: A biomarker of sperm maturity? Livestock Science. 250 , 104600
[Research article]

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Abstract

Breeding companies want to use semen from bulls as soon as possible to take advantage of their desirable genetics. It takes several weeks for the sperm quality of young bulls to stabilize and for post-thaw sperm quality to become acceptable for artificial insemination. Seminal plasma proteins protect spermatozoa during cryopreservation; it may take some time for the seminal plasma protein profile to stabilize. The purpose of this study was to determine if the seminal plasma protein profile can be used as a marker of likely seminal maturity in young bulls. A comparison was made of the seminal plasma protein profile in the ejaculates of 10 bulls of 9-10 months old (Sample I), with the profiles from ejaculates taken from the same bulls at 13-16 months old (Sample II) using fast protein liquid chromatography. This is a method for separating classes of proteins according to their binding ability. The peak area and peak height of different classes of proteins did not differ significantly between the two samples for each bull, except for peak 5 (heparin-binding proteins) and total peak area (p<0.05). The heparinbinding protein peak height and area were significantly higher (p<0.05) in Sample II than in Sample I. In conclusion, levels of fertility associated heparin-binding proteins increase with age in young bulls and might serve as a biomarker of sperm maturity. .

Authors/Creators:Deori, Sourabh and Hurri, Emma and Karkehabadi, Saeid and Morrell, Jane
Title:Fast protein liquid chromatography profiles of seminal plasma proteins in young bulls: A biomarker of sperm maturity?
Series Name/Journal:Livestock Science
Year of publishing :2021
Volume:250
Article number:104600
Number of Pages:6
Publisher:ELSEVIER
ISSN:1871-1413
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Genomic selection, Sperm quality, Fertility-associated proteins, Bovine semen
URN:NBN:urn:nbn:se:slu:epsilon-p-113217
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113217
Additional ID:
Type of IDID
DOI10.1016/j.livsci.2021.104600
Web of Science (WoS)000677697000008
ID Code:25118
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(VH) > Dept. of Clinical Sciences
(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:30 Aug 2021 21:25
Metadata Last Modified:31 Aug 2021 05:01

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