Deori, Sourabh and Hurri, Emma and Karkehabadi, Saeid and Morrell, Jane
(2021).
Fast protein liquid chromatography profiles of seminal plasma proteins in young bulls: A biomarker of sperm maturity?
Livestock Science. 250
, 104600
[Research article]
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Abstract
Breeding companies want to use semen from bulls as soon as possible to take advantage of their desirable genetics. It takes several weeks for the sperm quality of young bulls to stabilize and for post-thaw sperm quality to become acceptable for artificial insemination. Seminal plasma proteins protect spermatozoa during cryopreservation; it may take some time for the seminal plasma protein profile to stabilize. The purpose of this study was to determine if the seminal plasma protein profile can be used as a marker of likely seminal maturity in young bulls. A comparison was made of the seminal plasma protein profile in the ejaculates of 10 bulls of 9-10 months old (Sample I), with the profiles from ejaculates taken from the same bulls at 13-16 months old (Sample II) using fast protein liquid chromatography. This is a method for separating classes of proteins according to their binding ability. The peak area and peak height of different classes of proteins did not differ significantly between the two samples for each bull, except for peak 5 (heparin-binding proteins) and total peak area (p<0.05). The heparinbinding protein peak height and area were significantly higher (p<0.05) in Sample II than in Sample I. In conclusion, levels of fertility associated heparin-binding proteins increase with age in young bulls and might serve as a biomarker of sperm maturity. .
Authors/Creators: | Deori, Sourabh and Hurri, Emma and Karkehabadi, Saeid and Morrell, Jane | ||||||
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Title: | Fast protein liquid chromatography profiles of seminal plasma proteins in young bulls: A biomarker of sperm maturity? | ||||||
Series Name/Journal: | Livestock Science | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 250 | ||||||
Article number: | 104600 | ||||||
Number of Pages: | 6 | ||||||
Publisher: | ELSEVIER | ||||||
ISSN: | 1871-1413 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science. | ||||||
Keywords: | Genomic selection, Sperm quality, Fertility-associated proteins, Bovine semen | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-113217 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113217 | ||||||
Additional ID: |
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ID Code: | 25118 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (VH) > Dept. of Clinical Sciences (NL, NJ) > Department of Molecular Sciences | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 30 Aug 2021 21:25 | ||||||
Metadata Last Modified: | 31 Aug 2021 05:01 |
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