Li, Zhiqiang and Peirasmaki, Dimitra and Svard, Staffan and Abrink, Magnus and Åbrink, Magnus
(2021).
Serglycin-Deficiency Causes Reduced Weight Gain and Changed Intestinal Cytokine Responses in Mice Infected With Giardia intestinalis.
Frontiers in Immunology. 12
, 677722
[Research article]
![]() |
PDF
2MB |
Abstract
The proteoglycan serglycin (SG) is expressed by different innate and adaptive immune cells, e.g. mast cells, macrophages, neutrophils, and cytotoxic T lymphocytes, where SG contributes to correct granule storage and extracellular activity of inflammatory mediators. Here the serglycin-deficient (SG(-/-)) mouse strain was used to investigate the impact of SG on intestinal immune responses during infection with the non-invasive protozoan parasite Giardia intestinalis. Young (asymptotic to 11 weeks old) oral gavage-infected congenic SG(-/-) mice showed reduced weight gain as compared with the infected SG(+/+) littermate mice and the PBS-challenged SG(-/-) and SG(+/+) littermate mice. The infection caused no major morphological changes in the small intestine. However, a SG-independent increased goblet cell and granulocyte cell count was observed, which did not correlate with an increased myeloperoxidase or neutrophil elastase activity. Furthermore, infected mice showed increased serum IL-6 levels, with significantly reduced serum IL-6 levels in infected SG-deficient mice and decreased intestinal expression levels of IL-6 in the infected SG-deficient mice. In infected mice the qPCR analysis of alarmins, chemokines, cytokines, and nitric oxide synthases (NOS), showed that the SG-deficiency caused reduced intestinal expression levels of TNF-alpha and CXCL2, and increased IFN-gamma, CXCL1, and NOS1 levels as compared with SG-competent mice. This study shows that SG plays a regulatory role in intestinal immune responses, reflected by changes in chemokine and cytokine expression levels and a delayed weight gain in young SG(-/-) mice infected with G. intestinalis.
Authors/Creators: | Li, Zhiqiang and Peirasmaki, Dimitra and Svard, Staffan and Abrink, Magnus and Åbrink, Magnus | ||||||
---|---|---|---|---|---|---|---|
Title: | Serglycin-Deficiency Causes Reduced Weight Gain and Changed Intestinal Cytokine Responses in Mice Infected With Giardia intestinalis | ||||||
Series Name/Journal: | Frontiers in Immunology | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 12 | ||||||
Article number: | 677722 | ||||||
Number of Pages: | 10 | ||||||
Publisher: | FRONTIERS MEDIA SA | ||||||
ISSN: | 1664-3224 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Immunology in the medical area | ||||||
Keywords: | serglycin proteoglycan, knockout mouse, infection, Giardia intestinalis, innate intestinal immunity | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-113303 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113303 | ||||||
Additional ID: |
| ||||||
ID Code: | 25155 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Department of Biomedical Science and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 02 Sep 2021 11:26 | ||||||
Metadata Last Modified: | 02 Sep 2021 11:31 |
Repository Staff Only: item control page