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Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction

Givre, Lucas and Da Silva, Claire Crola and Swenson, Jon E. and Arnemo, Jon and Gauquelin-Koch, Guillemette and Bertile, Fabrice and Lefai, Etienne and Gomez, Ludovic (2021). Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction. Frontiers in Cardiovascular Medicine. 8 , 687501
[Research article]

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Abstract

Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury. Interestingly, despite physical inactivity during hibernation, brown bears (Ursus arctos) cope with cardiovascular physiological conditions that would be detrimental to humans. We hypothesized that bear serum might contain circulating factors that could provide protection against cell injury. In this study, we sought to determine whether addition of bear serum might improve cardiomyocyte survival following hypoxia-reoxygenation. Isolated mouse cardiomyocytes underwent 45 min of hypoxia followed by reoxygenation. At the onset of reoxygenation, cells received fetal bovine serum (FBS; positive control), summer (SBS) or winter bear serum (WBS), or adult serums of other species, as indicated. After 2 h of reoxygenation, propidium iodide staining was used to evaluate cell viability by flow cytometry. Whereas, 0.5% SBS tended to decrease reperfusion injury, 0.5% WBS significantly reduced cell death, averaging 74.04 +/- 7.06% vs. 79.20 +/- 6.53% in the FBS group. This cardioprotective effect was lost at 0.1%, became toxic above 5%, and was specific to the bear. Our results showed that bear serum exerts a therapeutic effect with an efficacy threshold, an optimal dose, and a toxic effect on cardiomyocyte viability after hypoxia-reoxygenation. Therefore, the bear serum may be a potential source for identifying new therapeutic molecules to fight against myocardial reperfusion injury and cell death in general.

Authors/Creators:Givre, Lucas and Da Silva, Claire Crola and Swenson, Jon E. and Arnemo, Jon and Gauquelin-Koch, Guillemette and Bertile, Fabrice and Lefai, Etienne and Gomez, Ludovic
Title:Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction
Series Name/Journal:Frontiers in Cardiovascular Medicine
Year of publishing :2021
Volume:8
Article number:687501
Number of Pages:8
Publisher:FRONTIERS MEDIA SA
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:cardiomyocyte, hypoxia-reoxygenation injury, protection, bear serum, hibernation, novel therapeutic strategy
URN:NBN:urn:nbn:se:slu:epsilon-p-113278
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113278
Additional ID:
Type of IDID
DOI10.3389/fcvm.2021.687501
Web of Science (WoS)000679963300001
ID Code:25179
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Wildlife, Fish and Environmental Studies
Deposited By: SLUpub Connector
Deposited On:03 Sep 2021 11:25
Metadata Last Modified:03 Sep 2021 11:31

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