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Characterization of Klebsiella pneumoniae complex isolates from pigs and humans in farms in Thailand: population genomic structure, antibiotic resistance and virulence genes

Leangapichart, Thongpan and Lunha, Kamonwan and Jiwakanon, Jatesada and Angkititrakul, Sunpetch and Jarhult, Josef D. and Magnusson, Ulf and Sunde, Marianne (2021). Characterization of Klebsiella pneumoniae complex isolates from pigs and humans in farms in Thailand: population genomic structure, antibiotic resistance and virulence genes. Journal of Antimicrobial Chemotherapy. 76 , 2012-2016
[Research article]

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Abstract

Objectives: To define characteristics of Klebsiella pneumoniae complex (hereafter KP) isolates from healthy pigs, farm workers and their householdmembers in Thailand.Methods: A total of 839 individual rectal swabs from pigs on 164 farms and 271 faecal samples of humans working on pig farms and persons living in the same household in Khon Kaen, Thailand were screened for gut colonization by KP. Genomic sequenceswere investigated for antibiotic resistance and virulence genes. Phylogenetic analyseswere performed in addition to comparison with isolates from previous studies from Thailand.Results: KP was detected in approximately 50% of pig and human samples. In total, 253 KP isolates were obtained: 39% from pigs, 34% from farmers and 26% from individuals living on the same farm but without animal contact. MLST revealed high genetic diversity with 196 different STs distributed over four phylogroups (Kp1 to Kp4). Low prevalence of ESBL-KP (7.5%) and colistin-resistant KP (3.2%) was observed among pigs and humans. Remarkably, four convergent MDR and hypervirulent strains were observed: one from pigs (ST290) and three from humans [ST35, ST3415 (strain 90CP1), ST17 (strain 90CM2)]. Sharing of KP clones among pigs and humans was identified for some STs including ST4788, ST661, ST3541 and ST29.Conclusions: The study indicated a low prevalence of ESBL and mcr genes among KP isolated from pigs and healthy humans in Thailand and suggested the possibility of zoonotic transmission for a subset of circulating KP clones.

Authors/Creators:Leangapichart, Thongpan and Lunha, Kamonwan and Jiwakanon, Jatesada and Angkititrakul, Sunpetch and Jarhult, Josef D. and Magnusson, Ulf and Sunde, Marianne
Title:Characterization of Klebsiella pneumoniae complex isolates from pigs and humans in farms in Thailand: population genomic structure, antibiotic resistance and virulence genes
Series Name/Journal:Journal of Antimicrobial Chemotherapy
Year of publishing :2021
Volume:76
Page range:2012-2016
Number of Pages:5
Publisher:OXFORD UNIV PRESS
ISSN:0305-7453
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Microbiology in the medical area
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
URN:NBN:urn:nbn:se:slu:epsilon-p-113423
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113423
Additional ID:
Type of IDID
DOI10.1093/jac/dkab118
Web of Science (WoS)000684142900008
ID Code:25265
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:07 Sep 2021 12:25
Metadata Last Modified:07 Sep 2021 12:31

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