Zahid, Muhammad Awais and Araujo Sandroni, Murilo and Vetukuri, Ramesh and Andreasson, Erik
(2021).
A fast, nondestructive method for the detection of disease-related lesions and wounded leaves.
Biotechniques. 71
[Research article]
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Abstract
Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.
| Authors/Creators: | Zahid, Muhammad Awais and Araujo Sandroni, Murilo and Vetukuri, Ramesh and Andreasson, Erik | ||||||
|---|---|---|---|---|---|---|---|
| Title: | A fast, nondestructive method for the detection of disease-related lesions and wounded leaves | ||||||
| Series Name/Journal: | Biotechniques | ||||||
| Year of publishing : | 2021 | ||||||
| Volume: | 71 | ||||||
| Number of Pages: | 6 | ||||||
| Publisher: | FUTURE SCI LTD | ||||||
| ISSN: | 0736-6205 | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||||
| Keywords: | image processing, leaf damage, lesions, phenotyping, plant disease, potato, Phytophthora | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-113434 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113434 | ||||||
| Additional ID: |
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| ID Code: | 25268 | ||||||
| Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||
| Department: | (LTJ, LTV) > Department of Plant Protection Biology (LTJ, LTV) > Department of Plant Breeding (from 130101) | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 07 Sep 2021 14:25 | ||||||
| Metadata Last Modified: | 07 Sep 2021 14:31 |
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