Home About Browse Search
Svenska


A fast, nondestructive method for the detection of disease-related lesions and wounded leaves

Zahid, Muhammad Awais and Araujo Sandroni, Murilo and Vetukuri, Ramesh and Andreasson, Erik (2021). A fast, nondestructive method for the detection of disease-related lesions and wounded leaves. Biotechniques. 71
[Research article]

[img] PDF
5MB

Abstract

Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.

Authors/Creators:Zahid, Muhammad Awais and Araujo Sandroni, Murilo and Vetukuri, Ramesh and Andreasson, Erik
Title:A fast, nondestructive method for the detection of disease-related lesions and wounded leaves
Series Name/Journal:Biotechniques
Year of publishing :2021
Volume:71
Number of Pages:6
Publisher:FUTURE SCI LTD
ISSN:0736-6205
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:image processing, leaf damage, lesions, phenotyping, plant disease, potato, Phytophthora
URN:NBN:urn:nbn:se:slu:epsilon-p-113434
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113434
Additional ID:
Type of IDID
DOI10.2144/btn-2021-0045
Web of Science (WoS)000684982400001
ID Code:25268
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:07 Sep 2021 14:25
Metadata Last Modified:07 Sep 2021 14:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits