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Evaluation of test characteristics of 2 ELISA tests applied to bulk tank milk and claw-trimming records for herd-level diagnosis of bovine digital dermatitis using latent class analysis

Holmoy, Ingrid H. and Ahlen, Lina and Frössling, Jenny and Sølverød, Liv and Holzhauer, Menno and Nodtvedt, Ane and Fjeldaas, Terje (2021). Evaluation of test characteristics of 2 ELISA tests applied to bulk tank milk and claw-trimming records for herd-level diagnosis of bovine digital dermatitis using latent class analysis. Journal of Dairy Science. 104 , 10111-10120
[Research article]

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Abstract

Bovine digital dermatitis (DD) is an infectious claw disease with a negative effect on animal welfare and production. Treponema spp. is the main causative agent, and infected animals produce specific antibodies. Our aim was to estimate sensitivity (Se) and specificity (Sp) of 2 ELISA research tests, Medicago's ELISA test and GD Animal Health's in-house ELISA test, for detection of DD-associated Treponema antibodies in bulk tank milk. We used bulk tank milk samples from 154 Nor-wegian dairy cattle herds, 96 from an expected high-prevalence region and 58 from a low-prevalence region. Both tests were evaluated separately against herd-level (aggregated) claw-trimming records extracted from the Norwegian Dairy Herd Recording System using latent class models in a Bayesian analysis. Cutoff values were selected using an explorative approach, and both non-informative priors for all parameters and informative beta priors for distribution of Se and Sp of claw trimming were explored. The estimated (median) true herd-level prevalence of digital dermatitis varied between 24 and 30% in the high-prevalence region and between 3 and 6% in the low-prevalence region. For Medicago's ELISA test, an Se (95% posterior credible interval) of 0.57 (0.32; 0.94) could be achieved without compromis -ing Sp, and for GD Animal Health's in-house ELISA test, an Se of 0.60 (0.37; 0.92) was achieved. Our study showed that both ELISA tests can detect antibodies against DD-associated Treponema spp. in bulk tank milk. However, neither of the 2 ELISA tests produced satisfactory sensitivity without compromising specific-ity. Based on these results, inspection at claw trimming in a chute is necessary for surveillance and control of DD at the herd level in Norway, although these ELISA tests of bulk tank milk might be a useful supplement.

Authors/Creators:Holmoy, Ingrid H. and Ahlen, Lina and Frössling, Jenny and Sølverød, Liv and Holzhauer, Menno and Nodtvedt, Ane and Fjeldaas, Terje
Title:Evaluation of test characteristics of 2 ELISA tests applied to bulk tank milk and claw-trimming records for herd-level diagnosis of bovine digital dermatitis using latent class analysis
Series Name/Journal:Journal of Dairy Science
Year of publishing :2021
Volume:104
Page range:10111-10120
Number of Pages:10
Publisher:ELSEVIER SCIENCE INC
ISSN:0022-0302
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Bayesian analysis, dairy cattle sensitivity, specificity, digital dermatitis
URN:NBN:urn:nbn:se:slu:epsilon-p-113480
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113480
Additional ID:
Type of IDID
DOI10.3168/jds.2020-19804
Web of Science (WoS)000687965500037
ID Code:25288
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Environment and Health
Deposited By: SLUpub Connector
Deposited On:09 Sep 2021 13:25
Metadata Last Modified:09 Sep 2021 13:31

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