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Non-enzymatic extraction of spermatozoa from alpaca ejaculates by pipetting followed by colloid centrifugation

Morrell, Jane and Karlsson Warring, Sofia and Norrestam, Emma and Malo, Clara and Huanca, Wilfredo (2021). Non-enzymatic extraction of spermatozoa from alpaca ejaculates by pipetting followed by colloid centrifugation. Livestock Science. 251 , 104627
[Research article]

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Abstract

Viscous camelid ejaculates present problems for sperm handling and sperm preservation. In the present study, a technique that had been used for dromedary camel semen was tested with alpaca semen. Ejaculates (n=9) were collected by artificial vagina at San Marcos University, Lima, and were liquefied by gentle pipetting in triscitrate-fructose. Half of the sample was prepared by Single Layer Centrifugation (SLC) through a colloid; the other half was centrifuged without colloid as a control. Each control and SLC sample was then split into two parts; one part was stored cooled for 24 h at 5 degrees C and the other part was frozen, resulting in 4 treatments for each ejaculate. All samples were evaluated for sperm motility, hypoosmotic swelling test (HOST), plasma membrane integrity, and morphology, immediately after centrifugation and again after storage Total motility and plasma membrane integrity were greater in samples prepared by SLC than controls (motility 72 +/- 13% vs. 57 +/- 7%; plasma membrane integrity 63 +/- 13% vs. 54 +/- 8%, for SLC and controls respectively). Normal morphology and HOST were not different between treatments (65 +/- 13 vs. 61 +/- 13% and 42 +/- 6 vs. 39 +/- 10%, for SLC and controls respectively). After 24 h cooled storage, motility and plasma membrane integrity were greater for SLC samples (motility: 51 +/- 16 vs. 34 +/- 15%; p<0.001; membrane integrity: 51 +/- 15 vs. 40 +/- 18%; P < 0.05 for SLC and controls, respectively); HOST (40 +/- 14 vs. 34 +/- 11%) and normal morphology (67 +/- 13 vs. 63 +/- 14%) were not different between treatments. Sperm quality decreased considerably after cryopreservation (P<0.001 for all parameters); however, motility (P<0.01), plasma membrane integrity (P<0.05) and morphology (P<0.05) were higher for SLC than for controls. These results indicate that alpaca spermatozoa can be extracted from semen using a combination of pipetting and SLC, potentially with a beneficial effect on sperm quality. Samples could be stored cooled for 24 h, retaining better motility than controls; motility and plasma membrane integrity were greater in SLC samples than controls after freezing and thawing but the freezing protocol requires improvement.

Authors/Creators:Morrell, Jane and Karlsson Warring, Sofia and Norrestam, Emma and Malo, Clara and Huanca, Wilfredo
Title:Non-enzymatic extraction of spermatozoa from alpaca ejaculates by pipetting followed by colloid centrifugation
Series Name/Journal:Livestock Science
Year of publishing :2021
Volume:251
Article number:104627
Number of Pages:7
Publisher:ELSEVIER
ISSN:1871-1413
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Silane silica-coated nanoparticles, South American camelids, Artificial insemination, Sperm quality, Sperm freezing
URN:NBN:urn:nbn:se:slu:epsilon-p-113484
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113484
Additional ID:
Type of IDID
DOI10.1016/j.livsci.2021.104627
Web of Science (WoS)000691527900020
ID Code:25295
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:10 Sep 2021 08:25
Metadata Last Modified:10 Sep 2021 08:31

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