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Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis

Gutierrez-Beltran, Emilio and Elander, Pernilla and Dalman, Kerstin and Dayhoff, Guy W. and Moschou, Panagiotis Nikolaou and Uversky, Vladimir N. and Crespo, Jose L. and Bozhkov, Peter (2021). Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. The Embo Journal. 40 , e105043
[Research article]

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Abstract

Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein-protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.

Authors/Creators:Gutierrez-Beltran, Emilio and Elander, Pernilla and Dalman, Kerstin and Dayhoff, Guy W. and Moschou, Panagiotis Nikolaou and Uversky, Vladimir N. and Crespo, Jose L. and Bozhkov, Peter
Title:Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
Series Name/Journal:The Embo Journal
Year of publishing :2021
Volume:40
Article number:e105043
Number of Pages:21
Publisher:WILEY
ISSN:0261-4189
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:heat stress, intrinsically disordered regions, SnRK1, SNF1, AMPK, stress granules, tudor staphylococcal nuclease
URN:NBN:urn:nbn:se:slu:epsilon-p-113227
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113227
Additional ID:
Type of IDID
DOI10.15252/embj.2020105043
Web of Science (WoS)000677815400001
ID Code:25312
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:13 Sep 2021 05:25
Metadata Last Modified:13 Sep 2021 05:31

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