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Optimization of Culture Conditions and Production of Bio-Fungicides from Trichoderma Species under Solid-State Fermentation Using Mathematical Modeling

Mulatu, Afrasa and Alemu, Tesfaye and Megersa, Negussie and Vetukuri, Ramesh (2021). Optimization of Culture Conditions and Production of Bio-Fungicides from Trichoderma Species under Solid-State Fermentation Using Mathematical Modeling. Microorganisms. 9 , 1675
[Research article]

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Abstract

Agro-industrial wastes suitable for economical and high mass production of novel Trichoderma species under solid-state fermentation were identified by optimizing the culture conditions using a mathematical model and evaluating the viability of the formulated bio-product. Fourteen inexpensive, locally available, organic substrates and cereals were examined using a one-factor-at-a-time experiment. The fungus colonized nearly all substrates after 21 days of incubation, although the degree of colonization and conidiation varied among the substrates. A mixture of wheat bran and white rice (2:1 w/w) was found to support maximum growth of T. asperellum AU131 (3.2 x 10(7) spores/g dry substrate) and T. longibrachiatum AU158 (3.5 x 10(7) spores/g dry substrate). Using a fractional factorial design, the most significant growth factors influencing biomass production were found to be temperature, moisture content, inoculum concentration, and incubation period (p <= 0.05). Analysis of variance of a Box-Behnken design showed that the regression model was highly significant (p <= 0.05) with F-values of 10.38 (P = 0.0027, T. asperellum AU131) and 12.01 (p < 0.0017, T. longibrachiatum AU158). Under optimal conditions, maximum conidia yield of log(10) (8.6) (T. asperellum AU131) and log(10)(9.18) (T. longibrachiatum) were obtained. For wettable powder Trichoderma species formulations, it was possible to maintain conidial viability at room temperature (25 degrees C) for eight months at concentrations above 10(6) CFU/g.

Authors/Creators:Mulatu, Afrasa and Alemu, Tesfaye and Megersa, Negussie and Vetukuri, Ramesh
Title:Optimization of Culture Conditions and Production of Bio-Fungicides from Trichoderma Species under Solid-State Fermentation Using Mathematical Modeling
Series Name/Journal:Microorganisms
Year of publishing :2021
Volume:9
Article number:1675
Number of Pages:25
Publisher:MDPI
ISSN:2076-2607
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 204 Chemical Engineering > Chemical Process Engineering
Keywords:agro-industrial waste, bioformulation, response surface methodology, viability, wheat bran
URN:NBN:urn:nbn:se:slu:epsilon-p-113546
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113546
Additional ID:
Type of IDID
DOI10.3390/microorganisms9081675
Web of Science (WoS)000690459900001
ID Code:25357
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:16 Sep 2021 09:25
Metadata Last Modified:16 Sep 2021 09:31

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