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Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen

Seyfferth, Carolin and Wessels, Bernard A. and Vahala, Jorma and Kangasjarvi, Jaakko and Delhomme, Nicolas and Hvidsten, Torgeir R. and Tuominen, Hannele and Lundberg-Felten, Judith (2021). Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen. Cells. 10 , 1971
[Research article]

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Abstract

Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus Ethylene Response Factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.

Authors/Creators:Seyfferth, Carolin and Wessels, Bernard A. and Vahala, Jorma and Kangasjarvi, Jaakko and Delhomme, Nicolas and Hvidsten, Torgeir R. and Tuominen, Hannele and Lundberg-Felten, Judith
Title:Populus PtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen
Series Name/Journal:Cells
Year of publishing :2021
Volume:10
Article number:1971
Number of Pages:19
Publisher:MDPI
ISSN:2073-4409
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Keywords:cell wall thickness, ERF85 (CRF4), lignin, ribosome biogenesis, wood development, xylem expansion
URN:NBN:urn:nbn:se:slu:epsilon-p-113580
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113580
Additional ID:
Type of IDID
DOI10.3390/cells10081971
Web of Science (WoS)000689001900001
ID Code:25368
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:16 Sep 2021 17:25
Metadata Last Modified:17 Sep 2021 05:01

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