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The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases

van der Woude, Lennard and Piotrowski, Markus and Klaasse, Gruson and Paulus, Judith K. and Krahn, Daniel and Ninck, Sabrina and Kaschani, Farnusch and Kaiser, Markus and Novak, Ondrej and Ljung, Karin and van Verk, Marcel and Snoek, Basten L. and Fiers, Martijn and Martin, Nathaniel I. and van Der Hoorn, Renier A. L. and Robert, Stephanie and Smeekens, Sjef and van Zanten, Martijn (2021). The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases. Plant Journal. 106 , 1523-1540
[Research article]

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Abstract

Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2-deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound 'Heatin', containing 1-iminomethyl-2-naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin-mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1-subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin-mediated hypocotyl elongation requires the NITRILASE1-subfamily members, NIT1 and NIT2. Heatin inhibits NIT1-subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1-subfamily substrate indole-3-acetonitrile in vivo. However, levels of the NIT1-subfamily product, bioactive auxin (indole-3-acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1-subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole-3-acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology.

Authors/Creators:van der Woude, Lennard and Piotrowski, Markus and Klaasse, Gruson and Paulus, Judith K. and Krahn, Daniel and Ninck, Sabrina and Kaschani, Farnusch and Kaiser, Markus and Novak, Ondrej and Ljung, Karin and van Verk, Marcel and Snoek, Basten L. and Fiers, Martijn and Martin, Nathaniel I. and van Der Hoorn, Renier A. L. and Robert, Stephanie and Smeekens, Sjef and van Zanten, Martijn
Title:The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases
Series Name/Journal:Plant Journal
Year of publishing :2021
Volume:106
Page range:1523-1540
Number of Pages:18
Publisher:WILEY
ISSN:0960-7412
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:chemical genetics, thermomorphogenesis, Arabidopsis, nitrilases, NIT1‐subfamily, Heatin, aldehyde oxidase, PIF4, 1‐iminomethyl‐2‐naphthol, IAN, indole‐3‐acetonitrile
URN:NBN:urn:nbn:se:slu:epsilon-p-111877
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111877
Additional ID:
Type of IDID
DOI10.1111/tpj.15250
Web of Science (WoS)000648013300001
ID Code:25483
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:22 Sep 2021 13:25
Metadata Last Modified:22 Sep 2021 13:31

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