van der Woude, Lennard and Piotrowski, Markus and Klaasse, Gruson and Paulus, Judith K. and Krahn, Daniel and Ninck, Sabrina and Kaschani, Farnusch and Kaiser, Markus and Novak, Ondrej and Ljung, Karin and van Verk, Marcel and Snoek, Basten L. and Fiers, Martijn and Martin, Nathaniel I. and van Der Hoorn, Renier A. L. and Robert, Stephanie and Smeekens, Sjef and van Zanten, Martijn
(2021).
The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases.
Plant Journal. 106
, 1523-1540
[Research article]
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Abstract
Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2-deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound 'Heatin', containing 1-iminomethyl-2-naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin-mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1-subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin-mediated hypocotyl elongation requires the NITRILASE1-subfamily members, NIT1 and NIT2. Heatin inhibits NIT1-subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1-subfamily substrate indole-3-acetonitrile in vivo. However, levels of the NIT1-subfamily product, bioactive auxin (indole-3-acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1-subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole-3-acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology.
Authors/Creators: | van der Woude, Lennard and Piotrowski, Markus and Klaasse, Gruson and Paulus, Judith K. and Krahn, Daniel and Ninck, Sabrina and Kaschani, Farnusch and Kaiser, Markus and Novak, Ondrej and Ljung, Karin and van Verk, Marcel and Snoek, Basten L. and Fiers, Martijn and Martin, Nathaniel I. and van Der Hoorn, Renier A. L. and Robert, Stephanie and Smeekens, Sjef and van Zanten, Martijn | ||||||
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Title: | The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases | ||||||
Series Name/Journal: | Plant Journal | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 106 | ||||||
Page range: | 1523-1540 | ||||||
Number of Pages: | 18 | ||||||
Publisher: | WILEY | ||||||
ISSN: | 0960-7412 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402) | ||||||
Keywords: | chemical genetics, thermomorphogenesis, Arabidopsis, nitrilases, NIT1‐subfamily, Heatin, aldehyde oxidase, PIF4, 1‐iminomethyl‐2‐naphthol, IAN, indole‐3‐acetonitrile | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-111877 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111877 | ||||||
Additional ID: |
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ID Code: | 25483 | ||||||
Faculty: | S - Faculty of Forest Sciences | ||||||
Department: | (S) > Dept. of Forest Genetics and Plant Physiology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 22 Sep 2021 13:25 | ||||||
Metadata Last Modified: | 22 Sep 2021 13:31 |
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