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An international inter-laboratory study on Nosema spp. spore detection and quantification through microscopic examination of crushed honey bee abdomens

Duquesne, Veronique and Gastaldi, Cristina and Del Cont, Aurelie and Cougoule, Nicolas and Bober, Andrzej and Brunain, Marleen and Chioveanu, Gabriela and Demicoli, Noel and Paulus, Petra Deakne and Somalo, Pilar Fernandez and Filipova, Miriam and Forsgren, Eva and Granato, Anna and Gurgulova, Kalinka and Heinikainen, Sirpa and Karssin, Age and Kinduriene, Irena and Koglberger, Hemma and Oureilidis, Konstantinos and Ozolina, Zanda and Pijacek, Martin and Ocepek, Metka Pislak and Schafer, Marc Oliver and Gajger, Ivana Tlak and Valerio, Maria Jose and Wakefield, Maureen and Franco, Stephanie (2021). An international inter-laboratory study on Nosema spp. spore detection and quantification through microscopic examination of crushed honey bee abdomens. Journal of Microbiological Methods. 184 , 106183
[Research article]

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Abstract

Nosemosis is a microsporidian disease causing mortality and weakening of honey bee colonies, especially in the event of co-exposure to other sources of stress. As a result, the disease is regulated in some countries. Reliable and harmonised diagnosis is crucial to ensure the quality of surveillance and research results. For this reason, the first European Interlaboratory Comparison (ILC) was organised in 2017 in order to assess both the methods and the results obtained by National Reference Laboratories (NRLs) in counting Nosema spp. spores by microscopy. Implementing their own routine conditions of analysis, the 23 participants were asked to perform an assay on & nbsp;a & nbsp;panel of ten positive and negative samples of crushed honey bee abdomens. They were asked to report results from a qualitative and quantitative standpoint. The assessment covered specificity, sensitivity, trueness and precision. Quantitative results were analysed in compliance with international standards NF ISO 13528 (2015) and NF ISO 5725-2 (1994). Three results showed a lack of precision and five a lack of trueness. However, overall results indicated a global specificity of 98% and a global sensitivity of 100%, thus demonstrating the advanced performance of the microscopic methods applied to Nosema spores by the NRLs. Therefore, the study concluded that using microscopy to detect and quantify spores of Nosema spp. was reliable and valid.panel of ten positive and negative samples of crushed honey bee abdomens. They were asked to report results from a qualitative and quantitative standpoint. The assessment covered specificity, sensitivity, trueness and precision. Quantitative results were analysed in compliance with international standards NF ISO 13528 (2015) and NF ISO 5725-2 (1994). Three results showed a lack of precision and five a lack of trueness. However, overall results indicated a global specificity of 98% and a global sensitivity of 100%, thus demonstrating the advanced performance of the microscopic methods applied to Nosema spores by the NRLs. Therefore, the study concluded

Authors/Creators:Duquesne, Veronique and Gastaldi, Cristina and Del Cont, Aurelie and Cougoule, Nicolas and Bober, Andrzej and Brunain, Marleen and Chioveanu, Gabriela and Demicoli, Noel and Paulus, Petra Deakne and Somalo, Pilar Fernandez and Filipova, Miriam and Forsgren, Eva and Granato, Anna and Gurgulova, Kalinka and Heinikainen, Sirpa and Karssin, Age and Kinduriene, Irena and Koglberger, Hemma and Oureilidis, Konstantinos and Ozolina, Zanda and Pijacek, Martin and Ocepek, Metka Pislak and Schafer, Marc Oliver and Gajger, Ivana Tlak and Valerio, Maria Jose and Wakefield, Maureen and Franco, Stephanie
Title:An international inter-laboratory study on Nosema spp. spore detection and quantification through microscopic examination of crushed honey bee abdomens
Series Name/Journal:Journal of Microbiological Methods
Year of publishing :2021
Volume:184
Article number:106183
Number of Pages:9
Publisher:ELSEVIER
ISSN:0167-7012
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Diagnosis, Interlaboratory comparison, Microscopic counting, Microsporidia
URN:NBN:urn:nbn:se:slu:epsilon-p-112026
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-112026
Additional ID:
Type of IDID
DOI10.1016/j.mimet.2021.106183
Web of Science (WoS)000641305800003
ID Code:25496
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:23 Sep 2021 13:34
Metadata Last Modified:23 Sep 2021 13:35

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