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New fluorescent auxin probes visualise tissue-specific and subcellular distributions of auxin in Arabidopsis

Parizkova, Barbora and Zukauskaite, Asta and Vain, Thomas and Grones, Peter and Raggi, Sara and Kubes, Martin F. and Kieffer, Martin and Doyle, Siamsa M. and Strnad, Miroslav and Kepinski, Stefan and Napier, Richard and Dolezal, Karel and Robert, Stephanie and Novak, Ondrej (2021). New fluorescent auxin probes visualise tissue-specific and subcellular distributions of auxin in Arabidopsis. New Phytologist. 230 , 535-549
[Research article]

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Abstract

In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. In this work, the introduction of a fluorophore to an auxin molecule represents a sensitive and non-invasive method to directly visualise auxin localisation with high spatiotemporal resolution.The state-of-the-art multidisciplinary approaches of genetic and chemical biology analysis together with live cell imaging, liquid chromatography-mass spectrometry (LC-MS) and surface plasmon resonance (SPR) methods were employed for the characterisation of auxin-related biological activity, distribution and stability of the presented compounds in Arabidopsis thaliana.Despite partial metabolisation in vivo, these fluorescent auxins display an uneven and dynamic distribution leading to the formation of fluorescence maxima in tissues known to concentrate natural auxin, such as the concave side of the apical hook. Importantly, their distribution is altered in response to different exogenous stimuli in both roots and shoots. Moreover, we characterised the subcellular localisation of the fluorescent auxin analogues as being present in the endoplasmic reticulum and endosomes.Our work provides powerful tools to visualise auxin distribution within different plant tissues at cellular or subcellular levels and in response to internal and environmental stimuli during plant development.

Authors/Creators:Parizkova, Barbora and Zukauskaite, Asta and Vain, Thomas and Grones, Peter and Raggi, Sara and Kubes, Martin F. and Kieffer, Martin and Doyle, Siamsa M. and Strnad, Miroslav and Kepinski, Stefan and Napier, Richard and Dolezal, Karel and Robert, Stephanie and Novak, Ondrej
Title:New fluorescent auxin probes visualise tissue-specific and subcellular distributions of auxin in Arabidopsis
Series Name/Journal:New Phytologist
Year of publishing :2021
Volume:230
Page range:535-549
Number of Pages:15
Publisher:WILEY
ISSN:0028-646X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Arabidopsis, distribution, fluorescent auxin, in vivo visualisation, subcellular localisation, transport
URN:NBN:urn:nbn:se:slu:epsilon-p-110935
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110935
Additional ID:
Type of IDID
DOI10.1111/nph.17183
Web of Science (WoS)000619213500001
ID Code:25515
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:27 Sep 2021 12:25
Metadata Last Modified:27 Sep 2021 12:31

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