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On the origin of the widespread self-compatible allotetraploid Capsella bursa-pastoris (Brassicaceae)

Bachmann, Jorg A. and Tedder, Andrew and Fracassetti, Marco and Steige, Kim A. and Lafon-Placette, Clément and Köhler, Claudia and Slotte, Tanja (2021). On the origin of the widespread self-compatible allotetraploid Capsella bursa-pastoris (Brassicaceae). Heredity. 127 , 124-134
[Research article]

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Abstract

Polyploidy, or whole-genome duplication, is a common speciation mechanism in plants. An important barrier to polyploid establishment is a lack of compatible mates. Because self-compatibility alleviates this problem, it has long been hypothesized that there should be an association between polyploidy and self-compatibility (SC), but empirical support for this prediction is mixed. Here, we investigate whether the molecular makeup of the Brassicaceae self-incompatibility (SI) system, and specifically dominance relationships among S-haplotypes mediated by small RNAs, could facilitate loss of SI in allopolyploid crucifers. We focus on the allotetraploid species Capsella bursa-pastoris, which formed similar to 300 kya by hybridization and whole-genome duplication involving progenitors from the lineages of Capsella orientalis and Capsella grandiflora. We conduct targeted long-read sequencing to assemble and analyze eight full-length S-locus haplotypes, representing both homeologous subgenomes of C. bursa-pastoris. We further analyze small RNA (sRNA) sequencing data from flower buds to identify candidate dominance modifiers. We find that C. orientalis-derived S-haplotypes of C. bursa-pastoris harbor truncated versions of the male SI specificity gene SCR and express a conserved sRNA-based candidate dominance modifier with a target in the C. grandiflora-derived S-haplotype. These results suggest that pollen-level dominance may have facilitated loss of SI in C. bursa-pastoris. Finally, we demonstrate that spontaneous somatic tetraploidization after a wide cross between C. orientalis and C. grandiflora can result in production of self-compatible tetraploid offspring. We discuss the implications of this finding on the mode of formation of this widespread weed.

Authors/Creators:Bachmann, Jorg A. and Tedder, Andrew and Fracassetti, Marco and Steige, Kim A. and Lafon-Placette, Clément and Köhler, Claudia and Slotte, Tanja
Title:On the origin of the widespread self-compatible allotetraploid Capsella bursa-pastoris (Brassicaceae)
Series Name/Journal:Heredity
Year of publishing :2021
Volume:127
Page range:124-134
Number of Pages:11
Publisher:SPRINGERNATURE
ISSN:0018-067X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
URN:NBN:urn:nbn:se:slu:epsilon-p-111815
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111815
Additional ID:
Type of IDID
DOI10.1038/s41437-021-00434-9
Web of Science (WoS)000641204000001
ID Code:25520
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:27 Sep 2021 13:02
Metadata Last Modified:27 Sep 2021 13:11

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