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Genomic Scan of Male Fertility Restoration Genes in a 'Gulzow' Type Hybrid Breeding System of Rye (Secale cereale L.)

Vendelbo, Nikolaj Meisner and Mahmood, Khalid and Sarup, Pernille and Kristensen, Peter Skov and Orabi, Jihad and Jahoor, Ahmed (2021). Genomic Scan of Male Fertility Restoration Genes in a 'Gulzow' Type Hybrid Breeding System of Rye (Secale cereale L.). International Journal of Molecular Sciences. 22 , 9277
[Research article]

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Abstract

Efficient and stable restoration of male fertility (Rf) is a prerequisite for large-scale hybrid seed production but remains an inherent issue in the predominant fertility control system of rye (Secale cereale L.). The 'Gulzow' (G)-type cytoplasmic male sterility (CMS) system in hybrid rye breeding exhibits a superior Rf. While having received little scientific attention, one major G-type Rf gene has been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosomes. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using recent advents in rye genomic resources. This includes: (I) genome-wide association studies (GWAS) on G-type germplasm; (II) GWAS on a biparental mapping population; and (III) an RNA sequence study to investigate the expression of genes residing in Rf-associated regions in G-type rye hybrids. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome belonging to the mitochondrial transcription termination factor gene family. We provisionally denote the identified novel Rf gene on 3RL RfNOS1. The discovery made in this study is distinct from known P- and C-type systems in rye as well as recognized CMS systems in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). We believe this study constitutes a stepping stone towards understanding the restoration of male fertility in the G-type CMS system and potential resources for addressing the inherent issues of the P-type system.

Authors/Creators:Vendelbo, Nikolaj Meisner and Mahmood, Khalid and Sarup, Pernille and Kristensen, Peter Skov and Orabi, Jihad and Jahoor, Ahmed
Title:Genomic Scan of Male Fertility Restoration Genes in a 'Gulzow' Type Hybrid Breeding System of Rye (Secale cereale L.)
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2021
Volume:22
Article number:9277
Number of Pages:16
Publisher:MDPI
ISSN:1661-6596
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:restoration of male fertility (Rf), cytoplasmic male sterility (CMS), pentatricopeptide repeat protein (PPR), mitochondrial transcription termination factor (mTERF), 600K SNP array, genome-wide association study (GWAS), RNAseq, chi-square, linkage disequilibrium
URN:NBN:urn:nbn:se:slu:epsilon-p-113711
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113711
Additional ID:
Type of IDID
DOI10.3390/ijms22179277
Web of Science (WoS)000694383500001
ID Code:25527
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:28 Sep 2021 09:35
Metadata Last Modified:28 Sep 2021 09:41

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