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Exploring the beta-tubulin gene family in a benzimidazole-resistant Parascaris univalens population

Martin, Frida and Halvarsson, Peter and Delhomme, Nicolas and Höglund, Johan and Tyden, Eva (2021). Exploring the beta-tubulin gene family in a benzimidazole-resistant Parascaris univalens population. International Journal for Parasitology: Drugs and Drug Resistance. 17 , 84-91
[Research article]

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Abstract

Benzimidazole (BZ) drugs are frequently used to treat infections with the equine ascarid Parascaris univalens due to increasing resistance to macrocyclic lactones and pyrantel. Benzimidazole resistance is rare in ascarids in contrast to strongyle parasites where this resistance is widespread. In strongyles, single nucleotide polymorphisms (SNPs) at codons 167, 198 and 200 in a 13-tubulin gene have been correlated to BZ resistance, but little is known about the 13-tubulin genes and their possible involvement in BZ resistance in P. univalens and other ascarids. Previously two 13-tubulin genes have been identified in P. univalens. In this study, we present five additional 13-tubulin genes as well as the phylogenetic relationship of all seven genes to 13-tubulins of other clade III and V nematodes. In addition, the efficacy of fenbendazole for treatment of P. univalens on a Swedish stud farm was studied in 2019 and 2020 using faecal egg count reduction test. Reductions varied from 73% to 88%, indicating the presence of a resistant P. univalens population on the farm. The emergence of BZ resistance emphasizes the need for development of molecular markers for rapid and more sensitive detection of resistant populations. We therefore investigated whether possible SNPs at positions 167, 198 or 200 in any of the 13-tubulin genes could be used to distinguish between resistant and susceptible P. univalens populations. Amplicon sequencing covering the mutation sites 167, 198 and 200 in all seven 13-tubulin genes revealed an absence of SNPs in both resistant and susceptible populations, suggesting that the mechanism behind BZ resistance in ascarids is different from that in strongyle nematodes and the search for a molecular marker for BZ resistance in P. univalens needs to continue.

Authors/Creators:Martin, Frida and Halvarsson, Peter and Delhomme, Nicolas and Höglund, Johan and Tyden, Eva
Title:Exploring the beta-tubulin gene family in a benzimidazole-resistant Parascaris univalens population
Series Name/Journal:International Journal for Parasitology: Drugs and Drug Resistance
Year of publishing :2021
Volume:17
Page range:84-91
Number of Pages:8
Publisher:ELSEVIER SCI LTD
ISSN:2211-3207
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Amplicon sequencing, Anthelmintic resistance, Equine, Fenbendazole
URN:NBN:urn:nbn:se:slu:epsilon-p-113745
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113745
Additional ID:
Type of IDID
DOI10.1016/j.ijpddr.2021.08.004
Web of Science (WoS)000697478600011
ID Code:25538
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
S - Faculty of Forest Sciences
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:30 Sep 2021 17:25
Metadata Last Modified:01 Oct 2021 05:01

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