Home About Browse Search
Svenska


Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress

Li, Xiang and Mao, Xiuhong and Xu, Yujin and Li, Yan and Zhao, Nan and Yao, Junxiu and Dong, Yufeng and Tigabu, Mulualem and Zhao, Xiyang and Li, Shanwen (2021). Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress. Ecotoxicology and Environmental Safety. 216 , 112179
[Research article]

[img] PDF
10MB

Abstract

Cadmium (Cd), a heavy metal element has strong toxicity to living organisms. Excessive Cd accumulation directly affects the absorption of mineral elements, inhibits plant tissue development, and even induces mortality. Populus × canadensis ‘Neva’, the main afforestation variety planted widely in northern China, was a candidate variety for phytoremediation. However, the genes relieving Cd toxicity and increasing Cd tolerance of this species were still unclear. In this study, we employed transcriptome sequencing on two Cd?treated cuttings to identify the key genes involved in Cd stress responses of P. × canadensis ‘Neva’ l induced by 0 (CK), 10 (C10), and 20 (C20) mg/L Cd(NO3)2 4H2O. We discovered a total of 2,656 (1,488 up-regulated and 1,168 downregulated) and 2,816 DEGs (1,470 up-regulated and 1,346 down-regulated) differentially expressed genes (DEGs) between the CK vs C10 and CK vs C20, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses in response to the Cd stress indicated that many DEGs identified were involved in the catalytic activity, the oxidoreductase activity, the transferase activity, and the biosynthesis of secondary metabolites. Based on the enrichment results, potential candidate genes were identified related to the calcium ion signal transduction, transcription factors, the antioxidant defense system, and transporters and showed divergent expression patterns under the Cd stress. We also validated the reliability of transcriptome data with the real-time PCR. Our findings deeper the understanding of the molecular responsive mechanisms of P. × canadensis ‘Neva’ lon Cd tolerance and further provide critical resources for phytoremediation applications.

Authors/Creators:Li, Xiang and Mao, Xiuhong and Xu, Yujin and Li, Yan and Zhao, Nan and Yao, Junxiu and Dong, Yufeng and Tigabu, Mulualem and Zhao, Xiyang and Li, Shanwen
Title:Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress
Series Name/Journal:Ecotoxicology and Environmental Safety
Year of publishing :2021
Volume:216
Article number:112179
Number of Pages:14
Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:0147-6513
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 405 Other Agricultural Sciences > Environmental Sciences related to Agriculture and Land-use
Keywords:Transcriptome, Cadmium stress, Physiological analysis, Transcription factors, Gene annotation
URN:NBN:urn:nbn:se:slu:epsilon-p-113737
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113737
Additional ID:
Type of IDID
DOI10.1016/j.ecoenv.2021.112179
Web of Science (WoS)000694855600009
ID Code:25558
Faculty:S - Faculty of Forest Sciences
Department:(S) > Southern Swedish Forest Research Centre
Deposited By: SLUpub Connector
Deposited On:05 Oct 2021 09:26
Metadata Last Modified:05 Oct 2021 09:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits