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A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

Rigal, Adeline and Doyle, Siamsa and Ritter, Andres and Raggi, Sara and Vain, Thomas and O'Brien, Jose Antonio and Goossens, Alain and Pauwels, Laurens and Robert, Stephanie (2021). A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception. Plant Physiology. 187 , 430-445
[Research article]

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Abstract

The plant hormone auxin, a master coordinator of development, regulates hypocotyl elongation during seedling growth. We previously identified the synthetic molecule RubNeddin 1 (RN1), which induces degradation of the AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors INDOLE-3-ACETIC ACID-INDUCIBLE3 (IAA3) and IAA7 in planta and strongly promotes hypocotyl elongation. In the present study, we show that despite the structural similarity of RN1 to the synthetic auxin 2,4-dichlorophenoxyacetic-acid (2,4-D), direct treatments with these compounds in Arabidopsis (Arabidopsis thaliana) result in distinct effects, possibly due to enhanced uptake of RN1 and low-level, chronic release of 2,4-D from RN1 in planta. We confirm RN1-induced hypocotyl elongation occurs via specific TRANSPORT INHIBITOR RESISTANT1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) receptor-mediated auxin signaling involving TIR1, AFB2, and AFB5. Using a transcriptome profiling strategy and candidate gene approach, we identify the genes ZINC FINGER OF ARABIDOPSIS THALIANA10 (ZAT10), ARABIDOPSIS TOXICOS EN LEVADURA31 (ATL31), and WRKY DNA-BINDING PROTEIN33 (WRKY33) as being rapidly upregulated by RN1, despite being downregulated by 2,4-D treatment. RN1-induced expression of these genes also occurs via TIR1/AFB-mediated auxin signaling. Our results suggest both hypocotyl elongation and transcription of these genes are induced by RN1 via the promoted degradation of the AUX/IAA transcriptional repressor IAA7. Moreover, these three genes, which are known to be stress-related, act in an inter-dependent transcriptional regulatory network controlling hypocotyl elongation. Together, our results suggest ZAT10, ATL31, and WRKY33 take part in a common gene network regulating hypocotyl elongation in Arabidopsis downstream of a selective auxin perception module likely involving TIR1, AFB2, and AFB5 and inducing the degradation of IAA7.

Authors/Creators:Rigal, Adeline and Doyle, Siamsa and Ritter, Andres and Raggi, Sara and Vain, Thomas and O'Brien, Jose Antonio and Goossens, Alain and Pauwels, Laurens and Robert, Stephanie
Title:A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception
Series Name/Journal:Plant Physiology
Year of publishing :2021
Volume:187
Page range:430-445
Number of Pages:16
Publisher:OXFORD UNIV PRESS INC
ISSN:0032-0889
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
URN:NBN:urn:nbn:se:slu:epsilon-p-113798
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113798
Additional ID:
Type of IDID
DOI10.1093/plphys/kiab269
Web of Science (WoS)000696244400039
ID Code:25561
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:05 Oct 2021 13:25
Metadata Last Modified:05 Oct 2021 13:31

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