Home About Browse Search
Svenska


Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells

Alim, Md Abdul and Grujic, Mirjana and Ackerman, Paul W. and Kristiansson, Per and Eliasson, Pernilla and Peterson, Magnus and Pejler, Gunnar (2021). Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells. Cellular and Molecular Immunology. 18 , 2383-2392
[Research article]

[img] PDF
3MB

Abstract

Mast cells are emerging as players in the communication between peripheral nerve endings and cells of the immune system. However, it is not clear the mechanism by which mast cells communicate with peripheral nerves. We previously found that mast cells located within healing tendons can express glutamate receptors, raising the possibility that mast cells may be sensitive to glutamate signaling. To evaluate this hypothesis, we stimulated primary mast cells with glutamate and showed that glutamate induced the profound upregulation of a panel of glutamate receptors of both the ionotropic type (NMDAR1, NMDAR2A, and NMDAR2B) and the metabotropic type (mGluR2 and mGluR7) at both the mRNA and protein levels. The binding of glutamate to glutamate receptors on the mast cell surface was confirmed. Further, glutamate had extensive effects on gene expression in the mast cells, including the upregulation of pro-inflammatory components such as IL-6 and CCL2. Glutamate also induced the upregulation of transcription factors, including Egr2, Egr3 and, in particular, FosB. The extensive induction of FosB was confirmed by immunofluorescence assessment. Glutamate receptor antagonists abrogated the responses of the mast cells to glutamate, supporting the supposition of a functional glutamate-glutamate receptor axis in mast cells. Finally, we provide in vivo evidence supporting a functional glutamate-glutamate receptor axis in the mast cells of injured tendons. Together, these findings establish glutamate as an effector of mast cell function, thereby introducing a novel principle for how cells in the immune system can communicate with nerve cells.

Authors/Creators:Alim, Md Abdul and Grujic, Mirjana and Ackerman, Paul W. and Kristiansson, Per and Eliasson, Pernilla and Peterson, Magnus and Pejler, Gunnar
Title:Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells
Series Name/Journal:Cellular and Molecular Immunology
Year of publishing :2021
Volume:18
Page range:2383-2392
Number of Pages:10
Publisher:NATURE PUBLISHING GROUP
ISSN:1672-7681
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Immunology in the medical area
Keywords:Glutamate, Glutamate receptors, Mast cells, NMDA receptors, Tryptase
URN:NBN:urn:nbn:se:slu:epsilon-p-105417
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105417
Additional ID:
Type of IDID
DOI10.1038/s41423-020-0421-z
Web of Science (WoS)000527501500001
ID Code:25854
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:18 Oct 2021 06:25
Metadata Last Modified:18 Oct 2021 06:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits