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Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa

Wijayawardene, Nalin N. and Bahram, Mohammad and Sanchez-Castro, Ivan and Dai, Dong-Qin and Ariyawansa, Kahandawa G. S. U. and Jayalal, Udeni and Suwannarach, Nakarin and Tedersoo, Leho (2021). Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa. Journal of Fungi. 7 , 703
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Abstract

Culture techniques are vital in both traditional and modern fungal taxonomy. Establishing sexual-asexual links and synanamorphs, extracting DNA and secondary metabolites are mainly based on cultures. However, it is widely accepted that a large number of species are not sporulating in nature while others cannot be cultured. Recent ecological studies based on culture-independent methods revealed these unculturable taxa, i.e., dark taxa. Recent fungal diversity estimation studies suggested that environmental sequencing plays a vital role in discovering missing species. However, Sanger sequencing is still the main approach in determining DNA sequences in culturable species. In this paper, we summarize culture-based and culture-independent methods in the study of ascomycetous taxa. High-throughput sequencing of leaf endophytes, leaf litter fungi and fungi in aquatic environments is important to determine dark taxa. Nevertheless, currently, naming dark taxa is not recognized by the ICN, thus provisional naming of them is essential as suggested by several studies.

Authors/Creators:Wijayawardene, Nalin N. and Bahram, Mohammad and Sanchez-Castro, Ivan and Dai, Dong-Qin and Ariyawansa, Kahandawa G. S. U. and Jayalal, Udeni and Suwannarach, Nakarin and Tedersoo, Leho
Title:Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa
Series Name/Journal:Journal of Fungi
Year of publishing :2021
Volume:7
Article number:703
Number of Pages:21
Publisher:MDPI
Language:English
Publication Type:Article Review/Survey
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biological Systematics
Keywords:consolidated species concept, fungal diversity, molecular taxonomy, morphological characters, traditional taxonomy, polyphasic approach
URN:NBN:urn:nbn:se:slu:epsilon-p-114016
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-114016
Additional ID:
Type of IDID
DOI10.3390/jof7090703
Web of Science (WoS)000699716700001
ID Code:25899
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:21 Oct 2021 13:25
Metadata Last Modified:21 Oct 2021 13:31

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