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Relationship of motility activation to lipid composition, protein profile, and swelling rate of burbot Lota lota spermatozoon following change of temperature and osmolality

Dadras, Hadiseh and Policar, Tomas and Loginov, Dmitry and Boryshpolets, Sergii and Loginova, Yana and Sampels, Sabine and Sotnikov, Anatolii and Kholodnyy, Vitaliy and Sterba, Jan and Xin, Miaomiao and Dzyuba, Borys (2021). Relationship of motility activation to lipid composition, protein profile, and swelling rate of burbot Lota lota spermatozoon following change of temperature and osmolality. Frontiers in marine science. 8 , 736087
[Research article]

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Abstract

Despite available information on the roles of osmotic pressure, potassium (K+), and calcium (Ca2+) in activation of Eurasian burbot spermatozoon motility, the changes in milt biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored spermatozoon swelling, lipid composition and proteome in relation to osmolality and temperature of swimming medium. The result revealed that temperature increment from 4 to 30°C increases burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca2+ decreases swelling of spermatozoa. No spermatozoon swelling was observed in non-ionic isotonic media at all studied temperatures. A role of swelling in activation of spermatozoa motility at 30°C was rejected. No differences were found in protein profile and lipid composition with respect to activation of burbot spermatozoa at 30°C. Burbot spermatozoon activation occurs at 30°C without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.

Authors/Creators:Dadras, Hadiseh and Policar, Tomas and Loginov, Dmitry and Boryshpolets, Sergii and Loginova, Yana and Sampels, Sabine and Sotnikov, Anatolii and Kholodnyy, Vitaliy and Sterba, Jan and Xin, Miaomiao and Dzyuba, Borys
Title:Relationship of motility activation to lipid composition, protein profile, and swelling rate of burbot Lota lota spermatozoon following change of temperature and osmolality
Series Name/Journal:Frontiers in marine science
Year of publishing :2021
Volume:8
Article number:736087
Number of Pages:14
ISSN:2296-7745
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-113936
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-113936
Additional ID:
Type of IDID
DOI10.3389/fmars.2021.736087
ID Code:25925
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:22 Oct 2021 10:25
Metadata Last Modified:22 Oct 2021 10:31

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